USER ORIENTATION, TRAINING AND SUPPORT FOR BIO-SAXS/D DATA ACQUISITION AND DATA

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 SSRL BTP工作人员为每个用户组提供有效使用SMB SAXS/D仪器的实践培训。所有用户首先收到针对BL4-2的简短安全指导和仪器概述，包括最近设备更改的更新。BTP工作人员讨论用户实验要求，根据需要配置仪器并培训用户。用户将收到用于在BL4-2上进行所有类型实验的Blu-Ice软件教程，其中特别强调特定的数据收集？选项卡？他们的数据收集需要，例如用于溶液散射研究的solSAXS选项卡。他们还接受有关样品处理设备的指导和关于SASTool(以前称为MarParse)的教程，SASTool是BTP工作人员最近开发的用于非晶体衍射研究的数据处理程序。用户在数据分析的初始步骤上得到指导，例如检查数据是否可能由辐射引起的聚合和评估数据质量。工作人员还可以帮助用户建立数据收集策略。由EMBL-Hamburg小组开发的数据处理程序Primus用于数据显示和计算基本结构参数，如回转半径(Rg)。BTP的工作人员通过一起测量第一组X射线散射数据来指导用户完成所有上述操作。工作人员还应要求提供关于欧洲同步辐射设施开发的Fit2D的简短教程。这些实践培训课程针对的是新手和有经验的用户群体。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The SSRL BTP staff provides each user group hands-on training on the effective use of the SMB SAXS/D instrument. All users first receive a brief safety orientation specific to BL4-2 and an overview of the instrument, including updates on recent equipment changes. The BTP staff discuss user experimental requirements, configure the instrument as necessary and train users. Users receive a tutorial on Blu-Ice software used for all types of experiment on BL4-2 with particular emphasis on a specific data collection ?tab? required for their data collection, for instance the solSAXS tab for solution scattering studies. They also receive instruction on sample-handling equipment and a tutorial on SAStool (formerly called MarParse), the data processing program for non-crystallined diffraction studies developed recently by the BTP staff. Users are instructed on initial steps of data analysis, such as inspecting data for possible radiation-induced aggregation and assessing data quality. The staff is also available to help users set up data collection strategies. The data processing program Primus, developed by an EMBL-Hamburg group, is used for data display and computing basic structural parameters such as radii of gyration (Rg). The BTP staff walks users through all the above by measuring first set of x-ray scattering data together. The staff also gives a short tutorial on Fit2D, developed at the European Synchrotron Radiation Facility, upon request. These hands-on training sessions are given to new as well as experienced user groups.