USER ORIENTATION, TRAINING AND SUPPORT FOR BIO-SAXS/D DATA ACQUISITION AND DATA

BIO-SAXS/D 数据采集和数据的用户指导、培训和支持

• 批准号: 8169962
• 负责人: THOMAS F WEISS
• 金额: $ 3.39万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169962
• 关键词: Computer Retrieval of Information on Scientific Projects Database; Computer software; Data; Data Analyses; Data Collection; Data Display; Data Quality; Equipment; European; Funding; Grant; Hand; Ice; Institution; Instruction; Measures; Radial; Radiation; Research; Research Personnel; Resources; Safety; Sampling; Solutions; Source; Training; Training Support; United States National Institutes of Health; Update; Walking; computerized data processing; data acquisition; experience; instrument; programs; research study; synchrotron radiation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The SSRL BTP staff provides each user group hands-on training on the effective use of the SMB SAXS/D instrument. All users first receive a brief safety orientation specific to BL4-2 and an overview of the instrument, including updates on recent equipment changes. The BTP staff discuss user experimental requirements, configure the instrument as necessary and train users. Users receive a tutorial on Blu-Ice software used for all types of experiment on BL4-2 with particular emphasis on a specific data collection ?tab? required for their data collection, for instance the solSAXS tab for solution scattering studies. They also receive instruction on sample-handling equipment and a tutorial on SAStool (formerly called MarParse), the data processing program for non-crystallined diffraction studies developed recently by the BTP staff. Users are instructed on initial steps of data analysis, such as inspecting data for possible radiation-induced aggregation and assessing data quality. The staff is also available to help users set up data collection strategies. The data processing program Primus, developed by an EMBL-Hamburg group, is used for data display and computing basic structural parameters such as radii of gyration (Rg). The BTP staff walks users through all the above by measuring first set of x-ray scattering data together. The staff also gives a short tutorial on Fit2D, developed at the European Synchrotron Radiation Facility, upon request. These hands-on training sessions are given to new as well as experienced user groups.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 SSRL BTP工作人员为每个用户组提供有关SMB SAXS/D仪器有效使用的实践培训。 所有用户首先会收到针对BL 4 -2的简要安全性说明和仪器概述，包括最近设备变更的更新。BTP工作人员讨论用户的实验要求，根据需要配置仪器并培训用户。用户会收到一份关于Blu-Ice软件的教程，该软件用于BL 4 -2上的所有类型的实验，特别强调特定的数据收集？标签？数据收集所需的软件，例如用于溶液散射研究的solSAXS选项卡。他们还接受了样品处理设备的指导和SAStool（以前称为MarParse）的教程，这是BTP工作人员最近开发的用于非晶体衍射研究的数据处理程序。向用户提供了数据分析初始步骤的指导，例如检查数据是否可能出现辐射引起的聚集现象和评估数据质量。工作人员还可以帮助用户制定数据收集战略。由EMBL-Hamburg小组开发的数据处理程序Primus用于数据显示和计算基本结构参数，如回转半径（Rg）。BTP工作人员通过测量第一组X射线散射数据，引导用户完成上述所有操作。工作人员还应要求提供关于Fit 2D的简短教程，Fit 2D是在欧洲同步辐射设施开发的。这些实践培训课程面向新用户和有经验的用户群体。