A NEW SAMPLE CHAMBER WITH TEMPERATURE AND HUMIDITY CONTROL FOR LIPID AND FIBER S

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 许多研究脂质、脂质-蛋白质相互作用和生物纤维的X射线实验需要能够准确监测和控制样品的温度和水合状态。在这些实验中，需要在各种不同的温度/水合条件下进行测量。在BL 4 -2上，一次测量的典型曝光时间范围从几秒到最多几分钟，因此为了有效利用实验时间，样品环境的控制不仅要准确，而且要合理快速。我们设计了一个新的样品室来满足这些要求，将Peltier元件与PID控制电子设备结合在一起，使我们能够快速改变并可靠地控制密封室内的温度和湿度条件。该腔室安装在标准测角器头上，测角器头通常由安装在BL 4 -2处的标准电动样品台上的Huber测角器固定。有了这个，我们可以很容易地改变倾斜角度的样品相对于光束在实验过程中，这是必要的实验上对齐的样品支撑在一个平面基板。PC通过串行端口（RS232）与控制电子设备通信，所有控制参数均可远程设置。该串行端口通信将允许我们在不久的将来将腔室控制集成到DCS/Blu-Ice光束线软件中。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Many x-ray experiments studying lipids, lipid-protein interactions and biological fibers require the ability to accurately monitor and control the temperature and hydration state of the specimen. In these experiments the measurements need to be done under a variety of different temperature/hydration conditions. On BL4-2 the typical exposure time for one measurement ranges from a few seconds to at most a few minutes, thus in order to make efficient use of the experimental time, the control of the sample environment should not only be accurate but also be reasonably fast. A new sample chamber has been designed to meet these requirements, incorporating Peltier elements together with PID control electronics, and allows us to quickly change and reliably control the temperature and humidity conditions inside the sealed chamber. The chamber is mounted on a standard goniometer head, which is usually held by a Huber goniostat mounted on the standard motorized sample stage at BL4-2. With this we can easily change the tilt angle of the sample with respect to the beam during the experiment which is necessary for experiments on aligned samples supported on a flat substrate. A PC is used to communicate with the control electronics through a serial port (RS232) and all the control parameters can be set remotely. This serial port communication will allow us in near future to integrate the chamber control into the DCS/Blu-Ice beam line software.