TRANSIENT STRUCTURAL STATES OF GROEL

GROEL 的瞬态结构状态

• 批准号: 7721719
• 负责人: ARTHUR L HORWICH
• 金额: $ 1.11万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-02-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721719
• 关键词: Aluminum; Arts; Carbon; Computer Retrieval of Information on Scientific Projects Database; Copper; Custom; Exposure to; Funding; Grant; Institution; Light; Photons; Reaction; Reporting; Research; Research Personnel; Resources; Solutions; Source; Temperature; Thick; Time; United States National Institutes of Health; absorption; abstracting; chaperonin; flash photolysis; photolysis

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. ABSTRACT A collaborative project (Art Horwich & George Farr, Yale) to investigate transient structural states of the chaperonin, GroEL, has commenced. This project will make use of the pre-mix/spray and flash-photolysis modes of time-resolved cryo-EM. We have described our implementation of flash-photolysis in previous reports. One problem that remains not fully resolved is the degree to which the temperature of the reaction solution rises upon exposure to a UV light flash which initiates photolysis of caged reactants. Currently, to minimize the temperature rise we use 50-um thick copper grids (custom made by Ladd Research) that are coated with aluminum and then covered with carbon nets to minimize absorption of photons by the carbon.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 摘要 一个合作项目（艺术Horwich和乔治法尔，耶鲁大学），调查瞬时结构状态的伴侣，GroEL，已经开始。本项目将利用时间分辨冷冻电镜的预混/喷雾和闪光光解模式。 我们在以前的报告中介绍了我们的闪光光解的实施情况。 一个仍然没有完全解决的问题是当暴露于引发笼状反应物光解的UV闪光时反应溶液的温度升高的程度。 目前，为了使温度升高最小化，我们使用50 μ m厚的铜网格（由拉德研究公司定制），这些网格涂有铝，然后覆盖有碳网，以使碳对光子的吸收最小化。