NOVEL SPLICING PROCESS OF A PROTEIN IN E COLI

大肠杆菌中蛋白质的新颖剪接过程

• 批准号: 7722287
• 负责人: Hanna Engelberg-Kulka
• 金额: $ 0.55万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722287
• 关键词: Amino Acids; Computer Retrieval of Information on Scientific Projects Database; Escherichia coli; Funding; Genes; Grant; Growth; Institution; Nature; Peptides; Phase; Process; Protein Engineering; Protein Splicing; Proteins; RNA; RNA Splicing; Reporting; Research; Research Personnel; Resources; Source; United States National Institutes of Health; intein; novel; size

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The linear expression of a given gene can be interrupted at two levels (RNA and protein) by unique segments of information encoded by the gene. Here we report on a novel splicing process of an engineered protein in Escherichia coli at the entry to stationary phase of growth. This process differs from the known protein splicing mechanism acting through inteins as follows: (a) the internal excised domain is a peptide, 21 amino acids long, that we call an "intide" (internal peptide); b) the nature of the amino acids inside the intide is not important, while the size of the intide is; (c) specific amino acids, i.e., RG upstream and KA downstream from the intide, have to be located 3 to 4 amino acids away from the borders of the intide; and (d) the process is growth-phase dependent.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 给定基因的线性表达可以在两个水平（RNA和蛋白质）被基因编码的独特信息片段中断。在这里，我们报告了一种新的剪接过程中的工程蛋白质在大肠杆菌进入稳定期的增长。该过程与已知的通过内含肽起作用的蛋白质剪接机制不同，如下：（a）内部切除结构域是21个氨基酸长的肽，我们称之为“内含肽”（内部肽）; B）内含肽内的氨基酸的性质不重要，而内含肽的大小重要;（c）特定氨基酸，即，RG上游和KA下游的内肽，必须位于3至4个氨基酸远离内肽的边界;和（d）该过程是生长期依赖性的。