mazEF-mediated programmed cell death network in E. coli
mazEF 介导的大肠杆菌程序性细胞死亡网络
基本信息
- 批准号:7469514
- 负责人:
- 金额:$ 15.12万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2007
- 资助国家:美国
- 起止时间:2007-07-15 至 2011-05-31
- 项目状态:已结题
- 来源:
- 关键词:AffectAntibioticsAntitoxinsApoptosisBacteriaBacterial ChromosomesBacteriophagesBehaviorBiochemicalBiochemical GeneticsBiological PhenomenaCell DeathCellsCessation of lifeChemicalsClassCleaved cellCommunicationConditionEscherichia coliEssential GenesFluorescence MicroscopyGene OrderGene ProteinsGenesGenetic ScreeningGenetic TranscriptionMediatingMembraneMethodsMicrobiologyOperonOrganismPeptide Signal SequencesPeptidesPhysiologicalPopulationProcessProductionPropertyProtein PrecursorsProteinsPublic HealthReportingResearchRoleSignal TransductionSignaling MoleculeSiteSpecific qualifier valueSystemToxinTranslationsWorkbasecell suicideextracellulargenetic regulatory proteininterestmutantnovelnovel strategiespreventprogramsquorum sensingreceptorresearch studyresponsethree dimensional structureyeast two hybrid system
项目摘要
DESCRIPTION (provided by applicant): Programmed cell death (PCD), defined as an active process which results in cell suicide, is recognized as an essential mechanism in multicellular organisms. In 1996, we reported on the first chromosomal toxin-antitoxin system discovered to be responsible for PCD in bacteria. This was the Escherichia coli (E. coli) mazEF regulateable module in which mazF encodes a stable toxin, MazF, and mazE encodes a labile antitoxin, MazE, that overcomes the lethal effect of MazF. Since 1996, we have made considerable progress in understanding the genetic, biochemical, structural and physiological properties of this system, and its relation to stressful conditions and the action of some well known antibiotics. In continuation of this work, here we propose to extend our research by a comprehensive characterization of a novel quorum sensing Extra-cellular Death Factor (EDF) that we were excited to discover as being a signaling peptide molecule required for E. coli mazEF-mediated cell death. Our five specific aims are as follows: 1 to characterize the exact chemical composition of E. coli EDF; 2. to elucidate the mechanism of EDF action; 3.To elucidate the relation of EDF to mazEF and to E. coli other toxin-antitoxin systems; 4.To characterize the EDF receiver(s) system; 5.To characterize EDFs of bacteria other than E. coli. The integrative research on EDF and its relation to bacterial death that we propose here should contribute to the understanding of a unique novel quorum sensing signaling molecule and its importance to bacterial PCD. Moreover, our results should also help to clarify a fundamental biological phenomenon of bacterial communication and thereby the multicellular behavior of bacterial populations. Practically, the results of our study may be useful as a basis for a novel strategy for generating a new class of antibiotics that trigger bacterial PCD, and thereby to help what has become a public health problem.
描述(由申请方提供):程序性细胞死亡(PCD)被定义为导致细胞自杀的主动过程,被认为是多细胞生物体的一种基本机制。1996年,我们报道了第一个染色体毒素-抗毒素系统被发现负责细菌中的PCD。这是大肠杆菌(E. coli)mazEF可调节模块,其中mazF编码稳定的毒素MazF,并且迷宫编码不稳定的抗毒素迷宫,其克服MazF的致死作用。自1996年以来,我们已经取得了相当大的进展,在了解这个系统的遗传,生化,结构和生理特性,以及它与压力条件和一些众所周知的抗生素的作用。在继续这项工作中,在这里,我们建议通过一种新的群体感应细胞外死亡因子(EDF)的全面表征来扩展我们的研究,我们很高兴地发现它是大肠杆菌所需的信号肽分子。coli mazEF介导的细胞死亡。本研究的主要目的如下:1.确定E. coli EDF; 2. 3.阐明EDF与MazEF、E. 4.鉴定EDF受体系统的特性; 5.鉴定除大肠杆菌外细菌的EDF。杆菌EDF及其与细菌死亡的关系的综合研究,我们建议在这里应该有助于了解一个独特的新的群体感应信号分子及其对细菌PCD的重要性。此外,我们的研究结果还有助于阐明细菌通讯的基本生物学现象,从而阐明细菌种群的多细胞行为。实际上,我们的研究结果可能是有用的基础上,为一种新的战略,产生一类新的抗生素,触发细菌PCD,从而帮助什么已经成为一个公共卫生问题。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Hanna Engelberg-Kulka其他文献
Hanna Engelberg-Kulka的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Hanna Engelberg-Kulka', 18)}}的其他基金
NOVEL SPLICING PROCESS OF A PROTEIN IN E COLI
大肠杆菌中蛋白质的新颖剪接过程
- 批准号:
8361540 - 财政年份:2011
- 资助金额:
$ 15.12万 - 项目类别:
NOVEL SPLICING PROCESS OF A PROTEIN IN E COLI
大肠杆菌中蛋白质的新颖剪接过程
- 批准号:
8169169 - 财政年份:2010
- 资助金额:
$ 15.12万 - 项目类别:
NOVEL SPLICING PROCESS OF A PROTEIN IN E COLI
大肠杆菌中蛋白质的新颖剪接过程
- 批准号:
7954138 - 财政年份:2009
- 资助金额:
$ 15.12万 - 项目类别:
NOVEL SPLICING PROCESS OF A PROTEIN IN E COLI
大肠杆菌中蛋白质的新颖剪接过程
- 批准号:
7722287 - 财政年份:2008
- 资助金额:
$ 15.12万 - 项目类别:
mazEF-mediated programmed cell death network in E. coli
mazEF 介导的大肠杆菌程序性细胞死亡网络
- 批准号:
7252773 - 财政年份:2007
- 资助金额:
$ 15.12万 - 项目类别:
mazEF-mediated programmed cell death network in E. coli
mazEF 介导的大肠杆菌程序性细胞死亡网络
- 批准号:
7622143 - 财政年份:2007
- 资助金额:
$ 15.12万 - 项目类别:
相似海外基金
Can antibiotics disrupt biogeochemical nitrogen cycling in the coastal ocean?
抗生素会破坏沿海海洋的生物地球化学氮循环吗?
- 批准号:
2902098 - 财政年份:2024
- 资助金额:
$ 15.12万 - 项目类别:
Studentship
The role of RNA repair in bacterial responses to translation-inhibiting antibiotics
RNA修复在细菌对翻译抑制抗生素的反应中的作用
- 批准号:
BB/Y004035/1 - 财政年份:2024
- 资助金额:
$ 15.12万 - 项目类别:
Research Grant
Metallo-Peptides: Arming Cyclic Peptide Antibiotics with New Weapons to Combat Antimicrobial Resistance
金属肽:用新武器武装环肽抗生素以对抗抗菌素耐药性
- 批准号:
EP/Z533026/1 - 财政年份:2024
- 资助金额:
$ 15.12万 - 项目类别:
Research Grant
Towards the sustainable discovery and development of new antibiotics
迈向新抗生素的可持续发现和开发
- 批准号:
FT230100468 - 财政年份:2024
- 资助金额:
$ 15.12万 - 项目类别:
ARC Future Fellowships
DYNBIOTICS - Understanding the dynamics of antibiotics transport in individual bacteria
DYNBIOTICS - 了解抗生素在单个细菌中转运的动态
- 批准号:
EP/Y023528/1 - 财政年份:2024
- 资助金额:
$ 15.12万 - 项目类别:
Research Grant
Engineering Streptomyces bacteria for the sustainable manufacture of antibiotics
工程化链霉菌用于抗生素的可持续生产
- 批准号:
BB/Y007611/1 - 财政年份:2024
- 资助金额:
$ 15.12万 - 项目类别:
Research Grant
The disulfide bond as a chemical tool in cyclic peptide antibiotics: engineering disulfide polymyxins and murepavadin
二硫键作为环肽抗生素的化学工具:工程化二硫多粘菌素和 murepavadin
- 批准号:
MR/Y033809/1 - 财政年份:2024
- 资助金额:
$ 15.12万 - 项目类别:
Research Grant
Role of phenotypic heterogeneity in mycobacterial persistence to antibiotics: Prospects for more effective treatment regimens
表型异质性在分枝杆菌对抗生素持久性中的作用:更有效治疗方案的前景
- 批准号:
494853 - 财政年份:2023
- 资助金额:
$ 15.12万 - 项目类别:
Operating Grants
Imbalance between cell biomass production and envelope biosynthesis underpins the bactericidal activity of cell wall -targeting antibiotics
细胞生物量产生和包膜生物合成之间的不平衡是细胞壁靶向抗生素杀菌活性的基础
- 批准号:
2884862 - 财政年份:2023
- 资助金额:
$ 15.12万 - 项目类别:
Studentship
Narrow spectrum antibiotics for the prevention and treatment of soft-rot plant disease
防治植物软腐病的窄谱抗生素
- 批准号:
2904356 - 财政年份:2023
- 资助金额:
$ 15.12万 - 项目类别:
Studentship