NOVEL SPLICING PROCESS OF A PROTEIN IN E COLI

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 特定基因的线性表达可以在两个水平(RNA和蛋白质)上被该基因编码的独特信息片段中断。在这里，我们报道了一种新的工程蛋白在大肠杆菌中的剪接过程，该剪接过程进入生长的静止阶段。这一过程不同于已知的通过内含子作用的蛋白质剪接机制：(A)内部切除的结构域是一个长21个氨基酸的肽，我们称之为“内潮”(内肽)；b)内潮内氨基酸的性质并不重要，而内潮的大小是重要的；(C)特定的氨基酸，即内潮上游的RG和下游的KA，必须位于离内潮边界3-4个氨基酸的位置；以及(D)这一过程取决于生长阶段。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The linear expression of a given gene can be interrupted at two levels (RNA and protein) by unique segments of information encoded by the gene. Here we report on a novel splicing process of an engineered protein in Escherichia coli at the entry to stationary phase of growth. This process differs from the known protein splicing mechanism acting through inteins as follows: (a) the internal excised domain is a peptide, 21 amino acids long, that we call an "intide" (internal peptide); b) the nature of the amino acids inside the intide is not important, while the size of the intide is; (c) specific amino acids, i.e., RG upstream and KA downstream from the intide, have to be located 3 to 4 amino acids away from the borders of the intide; and (d) the process is growth-phase dependent.