AURORA B PATHWAY PARTIALLY REGULATES SPINDLE ASSEMBLY
AURORA B PATHWAY PARTIALLY REGULATES SPINDLE ASSEMBLY
批准号:
7722250
负责人:
Hironori Funabiki
金额:
$0.33万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2008
资助国家:
美国
项目状态:
已结题
起止时间:
2008-03-01 至 2009-02-28
关键词:
AntibodiesBindingBypassChromatinComplexComputer Retrieval of Information on Scientific Projects DatabaseCytoplasmFundingGrantInstitutionMicrotubulesMitoticPathway interactionsPhosphoric Monoester HydrolasesPhosphotransferasesProcessProteinsRegulationResearchResearch PersonnelResourcesRunningSourceStructureUnited States National Institutes of HealthXenopusaurora B kinaseborealineggsurvivin
中文摘要
该子项目是利用
由NIH/NCRR资助的中心赠款提供的资源。子项目和
研究者(PI)可能从另一个NIH来源获得主要资金,
因此可以在其他CRISP条目中表示。列出的机构是
中心,不一定是研究者的机构。
染色质诱导的纺锤体组装依赖于染色体乘客复合物(CPC)对微管解聚蛋白的调节,所述CPC由Incexp、Survivin、Dasra(Borealin)和激酶Aurora B组成,但是Aurora B活性的空间调节的机制和意义尚不清楚。在这里,我们表明,极光B途径被抑制在非洲爪蟾卵提取物的细胞质中的磷酸酶,但它成为激活染色质通过RAN独立的机制。虽然纺锤体微管组装通常需要CPC的Dasra依赖性染色质结合,但Dasra的这种功能可以通过使用抗Incexp抗体聚集Aurora B-Incexp来绕过,该抗体刺激结合复合物之间的自激活。然而,这种染色质非依赖性极光B途径激活促进中心体微管组装,并产生异常的非染色体纺锤体样结构。我们建议,染色体富集的CPC的结果在当地的激酶自动激活,一种机制,有助于空间调控的纺锤体组装和可能的其他有丝分裂过程。
英文摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
Chromatin-induced spindle assembly depends on regulation of microtubule-depolymerizing proteins by the chromosomal passenger complex (CPC), consisting of Incenp, Survivin, Dasra (Borealin), and the kinase Aurora B, but the mechanism and significance of the spatial regulation of Aurora B activity remain unclear. Here, we show that the Aurora B pathway is suppressed in the cytoplasm of Xenopus egg extract by phosphatases, but that it becomes activated by chromatin via a Ran-independent mechanism. While spindle microtubule assembly normally requires Dasra-dependent chromatin binding of the CPC, this function of Dasra can be bypassed by clustering Aurora B-Incenp by using anti-Incenp antibodies, which stimulate autoactivation among bound complexes. However, such chromatin-independent Aurora B pathway activation promotes centrosomal microtubule assembly and produces aberrant achromosomal spindle-like structures. We propose that chromosomal enrichment of the CPC results in local kinase autoactivation, a mechanism that contributes to the spatial regulation of spindle assembly and possibly to other mitotic processes.
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Regulation of mitotic chromosomes
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批准号:10396982
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项目类别:
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资助金额:$83.06万
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财政年份:2019
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负责人:Hironori Funabiki
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依托单位:
Regulation of mitotic chromosomes
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Regulation of mitotic chromosomes
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批准号:8169194
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项目类别:
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项目类别:
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资助金额:$0.71万
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财政年份:2009
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依托单位:
AURORA B PATHWAY PARTIALLY REGULATES SPINDLE ASSEMBLY
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批准号:7954103
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项目类别:
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资助金额:$0.24万
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财政年份:2009
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负责人:Hironori Funabiki
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项目类别:
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Roles of chromosomal factors in chromosome segregation
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依托单位:
DNA DAMAGE REPAIR
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项目类别:
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资助金额:$0.36万
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财政年份:2005
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负责人:Hironori Funabiki
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依托单位:
Roles of chromosomal factors in chromosome segregation
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批准号:8111413
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项目类别:
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资助金额:$10.15万
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财政年份:2005
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依托单位:
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资助金额:$34.75万
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项目类别:
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资助金额:$34.75万
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依托单位:
Roles of chromosomal factors in chromosome segregation
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项目类别:
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资助金额:$30.45万
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财政年份:2005
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依托单位:
Roles of chromosomal factors in chromosome segregation
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批准号:8306799
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项目类别:
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资助金额:$34.75万
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财政年份:2005
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负责人:Hironori Funabiki
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依托单位:
Roles of chromosomal factors in chromosome segregation
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批准号:7098783
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项目类别:
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资助金额:$31.36万
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财政年份:2005
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负责人:Hironori Funabiki
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依托单位:
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