A QUANTITATIVE ANALYSIS SOFTWARE TOOL FOR MASS SPECTROMETRY-BASED PROTEOMICS

基于质谱的蛋白质组学定量分析软件工具

• 批准号: 7723617
• 负责人: John R Yates III
• 金额: $ 1万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7723617
• 关键词: Address; Algorithms; Censuses; Compatible; Computer Retrieval of Information on Scientific Projects Database; Data; Funding; Grant; Institution; Label; Mass Spectrum Analysis; Measurement; Proteomics; Research; Research Personnel; Resolution; Resources; Scanning; Software Tools; Source; Stable Isotope Labeling; Staging; Testing; United States National Institutes of Health; base; file format; improved; tandem mass spectrometry

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We describe Census, a quantitative software tool compatible with many labeling strategies as well as with label-free analyses, single-stage mass spectrometry (MS1) and tandem mass spectrometry (MS/MS) scans, and high- and low-resolution mass spectrometry data. Census uses robust algorithms to address poor-quality measurements and improve quantitative efficiency, and it can support several input file formats. We tested Census with stable-isotope labeling analyses as well as label-free analyses.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们描述了人口普查，一个定量的软件工具，兼容许多标记策略，以及与无标记的分析，单级质谱（MS 1）和串联质谱（MS/MS）扫描，高分辨率和低分辨率的质谱数据。Census使用强大的算法来解决低质量的测量并提高定量效率，它可以支持多种输入文件格式。我们用稳定同位素标记分析和无标记分析测试了普查。