QUANTITATIVE MASS SPECTROMETRY IDENTIFIES INSULIN SIGNALING TARGETS IN C ELEGAN

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 胰岛素受体β-2像蛋白质一样，调节秀丽隐杆线虫的代谢、发育和衰老。在定量蛋白质组学研究中，我们确定了86种蛋白质，它们在长寿daf-2突变体蠕虫中的丰度比野生型蠕虫中的丰度高或低。对这些蛋白质的一个子集的遗传研究表明，它们在一个或多个过程中起作用，包括进入dauer发育阶段和衰老。特别是，我们发现了一种补偿机制，激活响应减少的β 2信号，这涉及蛋白磷酸酶钙调磷酸酶。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DAF-2, an insulin receptor?like protein, regulates metabolism, development, and aging in Caenorhabditis elegans. In a quantitative proteomic study, we identified 86 proteins that were more or less abundant in long-lived daf-2 mutant worms than in wild-type worms. Genetic studies on a subset of these proteins indicated that they act in one or more processes regulated by DAF-2, including entry into the dauer developmental stage and aging. In particular, we discovered a compensatory mechanism activated in response to reduced DAF-2 signaling, which involves the protein phosphatase calcineurin.