SCREENING FOR BXI1P (BAX INHIBITOR-1) INTERACTING PROTEINS

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Bax Inhibitor-1 (BI-1) was first identified by its ability to block Bax-induced apoptotic cell death in the budding yeast, Saccharomyces cerevisiae. It is one member of the Bcl-2 family of proteins that regulates programmed cell death. Intriguingly, BI-1 is one of a few cell death inhibitors found in a wide range of fungi, plant, and animal species including Saccharomyces cerevisiae, Drosophila melanogaster, Arabidopsis thaliana, Mus musculus, and Homo sapiens. This suggests that it is an ancient protein. We are characterizing the yeast homolog of BI-1, which we are calling BXI1. Using a two-hybrid screen, we would like to identify proteins that interact with Bxi1p. With knowledge of the function of BXI1 in yeast we hope to better understand the function of Bax Inhibitor-1 in higher organisms including human beings.