SCREENING FOR BXI1P (BAX INHIBITOR-1) INTERACTING PROTEINS

筛选 BXI1P（BAX 抑制剂-1）相互作用蛋白

• 批准号: 7602136
• 负责人: NICANOR AUSTRIACO
• 金额: $ 1.04万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7602136
• 关键词: Animals; Apoptosis; Apoptotic; Cell Death; Computer Retrieval of Information on Scientific Projects Database; Drosophila melanogaster; Funding; Grant; Homo sapiens; Homologous Gene; House mice; Human; Institution; Knowledge; Mouse-ear Cress; Organism; Protein Family; Proteins; Range; Research; Research Personnel; Resources; Saccharomyces cerevisiae; Saccharomycetales; Screening procedure; Source; United States National Institutes of Health; Yeasts; inhibitor/antagonist; member; plant fungi; yeast two hybrid system

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Bax Inhibitor-1 (BI-1) was first identified by its ability to block Bax-induced apoptotic cell death in the budding yeast, Saccharomyces cerevisiae. It is one member of the Bcl-2 family of proteins that regulates programmed cell death. Intriguingly, BI-1 is one of a few cell death inhibitors found in a wide range of fungi, plant, and animal species including Saccharomyces cerevisiae, Drosophila melanogaster, Arabidopsis thaliana, Mus musculus, and Homo sapiens. This suggests that it is an ancient protein. We are characterizing the yeast homolog of BI-1, which we are calling BXI1. Using a two-hybrid screen, we would like to identify proteins that interact with Bxi1p. With knowledge of the function of BXI1 in yeast we hope to better understand the function of Bax Inhibitor-1 in higher organisms including human beings.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 Bax抑制剂-1（BI-1）首先通过其阻断芽殖酵母（酿酒酵母（Saccharomycescerevisiae））中Bax诱导的凋亡细胞死亡的能力而被鉴定。 它是调节程序性细胞死亡的Bcl-2蛋白家族的成员之一。 有趣的是，BI-1是在广泛的真菌、植物和动物物种中发现的少数细胞死亡抑制剂之一，包括酿酒酵母、黑腹果蝇、拟南芥、小家鼠和智人。这表明它是一种古老的蛋白质。我们正在表征BI-1的酵母同系物，我们称之为BXI 1。 使用双杂交筛选，我们希望鉴定与Bxi 1 p相互作用的蛋白质。 通过对BXI 1在酵母中功能的了解，我们希望更好地了解Bax Inhibitor-1在包括人类在内的高等生物中的功能。