NEUROCHEMICAL PROFILES OF RODENTS MEASURED AT 94 T

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 广泛的脑代谢物（神经化学谱）的可靠定量显著增加了从光谱数据中提取的神经化学信息，这允许在监测脑生物化学方面有非常具体的观点。使用超短回波时间STEAM定位序列（TE = 2 ms）和LCModel数据分析开发的方法用于各种合作项目，重点是大脑发育和神经退行性变的动物模型。在缺氧/缺血动物模型（Rao et al. 2007）和新生儿缺铁模型（Ward et al. 2007）中研究了神经化学特征的变化。该方法还应用于亨廷顿病的转基因小鼠模型，其揭示了疾病进展中的多种神经化学过程（Tkac等人，2007）。由于总肌酸水平的显著变化，使用未抑制的水信号的绝对代谢物定量对于测量数据的正确生化解释至关重要。9.4 T的光谱学用于研究冬眠期间的神经化学过程（地松鼠）（亨利等人，2007年）。这项研究证明了在非常低的温度（~ 50 ℃）下进行高分辨光谱分析的可行性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Reliable quantification of a broad range of brain metabolites (neurochemical profile) significantly increases the neurochemical information extractable from spectroscopy data, which allows very specific view in monitoring brain biochemistry. Developed methodology using ultra-short echo-time STEAM localization sequence (TE = 2 ms) and LCModel data analysis was used in variety of collaborative projects focused on animal models of the brain development and neurodegeneration. Changes in the neurochemical profile were studied in an animal model of hypoxia/ischemia (Rao et al. 2007) and model for neonatal iron deficiency (Ward et al. 2007). This methodology was also applied in the transgenic mouse model of Huntington's disease, which revealed multiple neurochemical processes in progression of the diseases (Tkac et al. 2007). The absolute metabolite quantification using unsuppressed water signal was crucial for correct biochemical interpretation of measured data due to significant changes in total creatine levels. Spectroscopy at 9.4 T was used in the study of the neurochemical processes during hibernation (ground squirrels) (Henry et al. 2007). This study demonstrated the feasibility of highly resolved spectroscopy at very low temperatures (~ 50C).