NEUROCHEMICAL PROFILES OF RODENTS MEASURED AT 94 T

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Reliable quantification of a broad range of brain metabolites (neurochemical profile) significantly increases the neurochemical information extractable from spectroscopy data, which allows very specific view in monitoring brain biochemistry. Developed methodology using ultra-short echo-time STEAM localization sequence (TE = 2 ms) and LCModel data analysis was used in variety of collaborative projects focused on animal models of the brain development and neurodegeneration. Changes in the neurochemical profile were studied in an animal model of hypoxia/ischemia (Rao et al. 2007) and model for neonatal iron deficiency (Ward et al. 2007). This methodology was also applied in the transgenic mouse model of Huntington's disease, which revealed multiple neurochemical processes in progression of the diseases (Tkac et al. 2007). The absolute metabolite quantification using unsuppressed water signal was crucial for correct biochemical interpretation of measured data due to significant changes in total creatine levels. Spectroscopy at 9.4 T was used in the study of the neurochemical processes during hibernation (ground squirrels) (Henry et al. 2007). This study demonstrated the feasibility of highly resolved spectroscopy at very low temperatures (~ 50C).