ES CELL CULTURE PROGRAM: MOUSE ES CELLS: GLYCOSYLATION PATTERNS OF GLYCOLIPIDS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在目前的研究中，我们正在表征小鼠胚胎干细胞的凝集素结合特性和 描述在分化过程中发生的结合的变化。这一分析正被扩展到分析 糖脂及其在相同条件下的糖基化。这项工作的一个主要目标是定义细胞 在mESCs表面的表面表位可以改进对多能状态的分析并改进 早期分化事件的分析。目前用于确定mESCs及其分化的细胞表面标志物 导数的用途有限，因为它们的表达没有严格限制到特定的细胞状态，并且因为 其表现的变化显示出与分化事件相关的较差的时间分辨率。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In the present study we are characterizing the lectin binding properties of mouse embryonic stem cells and describing changes in binding that occur during differentiation. This analysis is being extended to the analysis of glycolipids and their glycosylation under the same conditions. One major objective of this work is to define cell surface epitopes on the surface of mESCs that can improve the analysis of the pluripotent state and to improve the analysis of early differentiation events. Current cell surface markers used to define mESCs and their differentiated derivatives have limited utility as their expression is not tightly restricted to a particular cell state and because changes in their presentation show poor temporal resolution in relation to differentiation events.