ES CELL CULTURE PROGRAM: MOUSE ES CELLS: GLYCOSYLATION PATTERNS OF GLYCOLIPIDS

ES 细胞培养程序：小鼠 ES 细胞：糖脂的糖基化模式

• 批准号: 7722612
• 负责人: Stephen Dalton
• 金额: $ 11.28万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-08 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722612
• 关键词: Binding; Cell surface; Cells; Computer Retrieval of Information on Scientific Projects Database; Condition; Cultured Cells; Epitopes; Event; Funding; Glycolipids; Grant; Institution; Lectin; Mus; Pattern; Property; Research; Research Personnel; Resolution; Resources; Source; Surface; United States National Institutes of Health; Work; embryonic stem cell; glycosylation; improved; programs

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In the present study we are characterizing the lectin binding properties of mouse embryonic stem cells and describing changes in binding that occur during differentiation. This analysis is being extended to the analysis of glycolipids and their glycosylation under the same conditions. One major objective of this work is to define cell surface epitopes on the surface of mESCs that can improve the analysis of the pluripotent state and to improve the analysis of early differentiation events. Current cell surface markers used to define mESCs and their differentiated derivatives have limited utility as their expression is not tightly restricted to a particular cell state and because changes in their presentation show poor temporal resolution in relation to differentiation events.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在本研究中，我们表征了小鼠胚胎干细胞的凝集素结合特性， 描述了在分化过程中发生的结合变化。这一分析正在扩大到分析 糖脂及其糖基化在相同条件下。这项工作的一个主要目标是定义细胞 mESC表面上的表面表位，其可以改善对多能状态的分析并改善多能性。 早期分化事件的分析。 目前用于定义mESC及其分化的细胞表面标志物 衍生物的效用有限，因为它们的表达不严格限于特定的细胞状态， 它们表现的变化显示出与分化事件相关的较差的时间分辨率。