DISTRIBUTION OF HUMAN PLURIPOTENT STEM CELLS

人类多能干细胞的分布

• 批准号: 8363004
• 负责人: Stephen Dalton
• 金额: $ 10.32万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363004
• 关键词: Cell Culture Techniques; Cells; Flow Cytometry; Funding; Grant; Human; National Center for Research Resources; Pluripotent Stem Cells; Principal Investigator; Quality Control; RNA; Research; Research Infrastructure; Resources; Role; Source; Staining method; Stains; Technology; United States National Institutes of Health; cost; human embryonic stem cell; induced pluripotent stem cell

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A major role of Core 1 of this Resource is to grow and quality control human pluripotent stem cells. Pluripotent cells are cultured in chemically defined media and quality controlled by immuno-staining, Q-PCR (RNA) and flow cytometry analysis. Pluripotent cells grown by the Core include human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs).

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 该资源的核心1的主要作用是培养和质量控制人类多能干细胞。在化学成分确定的培养基中培养多能细胞，并通过免疫染色、Q-PCR（RNA）和流式细胞术分析进行质量控制。 Core培养的多能细胞包括人胚胎干细胞（hESC）和人诱导多能干细胞（hiPSC）。