CELL SURFACE GLYCOSPHINGOLIPIDS SSEA-3 AND SSEA-4

细胞表面糖脂 SSEA-3 和 SSEA-4

• 批准号: 7722618
• 负责人: ALFRED Harrison MERRILL
• 金额: $ 0.94万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-08 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722618
• 关键词: Carbohydrates; Cell surface; Cells; Computer Retrieval of Information on Scientific Projects Database; Development; Epitopes; Funding; Grant; Human; Human Development; In Vitro; Institution; Modeling; Molecular; Population; Proteoglycan; Research; Research Personnel; Resources; Source; Sphingolipids; Staging; Testing; Undifferentiated; United States National Institutes of Health; blastocyst; embryonic antigen; embryonic stem cell; extracellular; stage-specific embryonic antigen 4

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Pluripotent cells can be isolated from the human blastocyst and maintained in culture as self-renewing, undifferentiated, embryonic stem cells (hESCs). They are a valuable model of human development in vitro, and are the focus of substantial research aimed at generating differentiated populations for cellular therapies. The extracellular markers that have been used to characterize hESCs are primarily carbohydrate epitopes on proteoglycans or sphingolipids, such as the stage-specific embryonic antigens (SSEA)-3 and 4. The expression of SSEA-3 and 4 is tightly regulated during preimplantation development and on hESCs. While this might imply a molecular function in undifferentiated cells, it has not yet been tested experimentally.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 多能细胞可以从人胚泡中分离出来，并作为自我更新细胞维持在培养物中， 未分化的胚胎干细胞（hESC）。它们是体外人类发育的宝贵模型， 实质性研究的焦点在于产生用于细胞疗法的分化群体。的 已经用于表征hESC的细胞外标记物主要是hESC上的碳水化合物表位。 蛋白聚糖或鞘脂，如阶段特异性胚胎抗原（SSEA）-3和 4.的表达 SSEA-3及 4在植入前发育期间和hESC上受到严格调控。虽然这可能意味着 在未分化细胞中的分子功能，它还没有经过实验测试。