CELL SURFACE GLYCOSPHINGOLIPIDS SSEA-3 AND SSEA-4

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 多能细胞可以从人类胚泡中分离出来，并在培养中保持自我更新，未分化， 胚胎干细胞(HESCs)。它们是人类体外发育的有价值的模型，也是实质性研究的焦点。 旨在为细胞疗法产生差异化群体的研究。已经存在的细胞外标志物 用于表征hESCs的主要是蛋白多糖或鞘脂上的碳水化合物表位，例如阶段- 胚胎特异性抗原(SSEA)-3和4。SSEA-3和SSEA-4的表达在着床前受到严格调控 发展和人类可持续发展。虽然这可能意味着未分化细胞的分子功能，但它还没有 经过实验测试。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Pluripotent cells can be isolated from the human blastocyst and maintained in culture as self-renewing, undifferentiated, embryonic stem cells (hESCs). They are a valuable model of human development in vitro, and are the focus of substantial research aimed at generating differentiated populations for cellular therapies. The extracellular markers that have been used to characterize hESCs are primarily carbohydrate epitopes on proteoglycans or sphingolipids, such as the stage- specific embryonic antigens (SSEA)-3 and 4. The expression of SSEA-3 and 4 is tightly regulated during preimplantation development and on hESCs. While this might imply a molecular function in undifferentiated cells, it has not yet been tested experimentally.