CELL SURFACE GLYCOSPHINGOLIPIDS SSEA-3 AND SSEA-4

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 多能细胞可以从人胚泡中分离出来，并以自我更新、未分化、 胚胎干细胞（hESC）。它们是体外人类发育的有价值的模型，并且是实质性研究的焦点。 旨在为细胞疗法产生分化群体的研究。细胞外标记物 用于表征hESC的主要是蛋白聚糖或鞘脂上的碳水化合物表位，例如阶段- 特异性胚胎抗原（SSEA）-3和 4. SSEA-3的表达和 4在植入前受到严格调节 开发和hESC。虽然这可能意味着在未分化细胞中的分子功能，但它还没有被证实。 实验测试。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Pluripotent cells can be isolated from the human blastocyst and maintained in culture as self-renewing, undifferentiated, embryonic stem cells (hESCs). They are a valuable model of human development in vitro, and are the focus of substantial research aimed at generating differentiated populations for cellular therapies. The extracellular markers that have been used to characterize hESCs are primarily carbohydrate epitopes on proteoglycans or sphingolipids, such as the stage- specific embryonic antigens (SSEA)-3 and 4. The expression of SSEA-3 and 4 is tightly regulated during preimplantation development and on hESCs. While this might imply a molecular function in undifferentiated cells, it has not yet been tested experimentally.