权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Molecular Insight into Polyaromatic Toxicant Degradation by Microbial Communities

微生物群落对多环芳烃有毒物质降解的分子洞察

基本信息

批准号：
7599128
负责人：
JAMES M TIEDJE
金额：
$ 45.06万
依托单位：
MICHIGAN STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7599128
关键词：
Academy Address Aerobic American Aromatic Hydrocarbons Aromatic Polycyclic Hydrocarbons Biochemical Pathway Biodegradation Biological Markers Bioremediations Burkholderia Cataloging Catalogs Categories Characteristics Chemicals Cloning Communities Complement DNA Dependence Diagnostic Dioxins Drug Metabolic Detoxication Ecology Environment Environmental Microbiology Enzymes Equipment and supply inventories Evolution Fingerprint Gene Family Genes Genetic Variation Genomics Goals Intervention Knowledge Libraries Measures Metabolic Metagenomics Methods Michigan Microarray Analysis Microbiology Modeling Molecular Molecular Analysis Molecular Biology Monitor Nature Operon Organism Oxygenases Pathway interactions Personal Satisfaction Physiology Polychlorinated Biphenyls Polymerase Chain Reaction Population Rate Recovery Reporting Sampling Scientist Screening procedure Site Soil Stress System Systems Biology Techniques Technology Time Toxicogenomics Universities Ursidae Family base concept dechlorination dibenzofuran genome sequencing high throughput screening insight interest metabolic abnormality assessment microbial microbial community pollutant remediation research study stable isotope superfund chemical tool toxicant

项目摘要

The microbial world is diverse owing to its 3.7 billion years of evolution, which provides for both the opportunity of undiscovered metabolic capacity, including that for pollutant degradation, and the challenge of detecting and recovering this activity. It is well known that more than 99% of the microbial world has not been cultured and hence remains undiscovered. We propose to explore and recover genes for two key biodegradative steps in the detoxification of chlorinated polyaromatic compounds from the DNA of this uncultured microbial diversity, and then to use the molecular markers from this study to aid in site assessment and quantitative predictions of biodegradation at contaminated sites. We are targeting the reductive dehalogenases and the aromatic oxygenases as the key functions to recover since they are most often the rate limiting steps in the degradation of the polychlorinated dioxins and dibenzofurans, PCBs and polynuclear aromatic hydrocarbons (PAHs), the Superfund chemicals of focus in our study. Our specific aims are to: (1) explore and recover nature's catalytic diversity with the goal of developing a comprehensive profile of microbial metabolic capabilities for these polyaromatic compounds, (2) use the genome sequence information of Burkholderia xenovorans LB400, the most effective PCB degrader, to study the metabolic features important to the degradation of these chemicals and (3), develop quantitative diagnostic tools based on Bayesian probabilistic networks to predict biodegradation at contaminated sites. We propose to use enrichments to help identify the functionally active populations and hence genes, to use stable isotope probing to recover DNA from the active degrading populations, to use metagenomic libraries to recover the full genes and operons, and to use high throughput PCR screening for identifying clones with the targeted gene families. This project combines the expertise of the Rutgers University scientists in aromatic oxygenases and high throughput screening and metagenomics with the expertise at Michigan State University in reductive dechlorination and genomics and microarray technology.

微生物世界是多样化的，因为它有37亿年的进化，这提供了两个