Molecular Insight into Polyaromatic Toxicant Degradation by Microbial Communities
微生物群落对多环芳烃有毒物质降解的分子洞察
基本信息
- 批准号:8055596
- 负责人:
- 金额:$ 43.56万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-04-01 至 2013-03-31
- 项目状态:已结题
- 来源:
- 关键词:AcademyAddressAerobicAmericanAromatic HydrocarbonsAromatic Polycyclic HydrocarbonsBiochemical PathwayBiodegradationBioremediationsBurkholderiaCatalogingCatalogsCategoriesCharacteristicsChemicalsCloningCommunitiesComplementDNADependenceDiagnosticDioxinsDrug Metabolic DetoxicationEcologyEnvironmentEnvironmental MicrobiologyEnzymesEquipment and supply inventoriesEvolutionFingerprintGene FamilyGenesGenetic VariationGenomicsGoalsInterventionKnowledgeLibrariesMeasuresMetabolicMetagenomicsMethodsMichiganMicroarray AnalysisMicrobiologyModelingMolecularMolecular AnalysisMolecular BiologyMonitorNatureOperonOrganismOxygenasesPathway interactionsPhysiologyPolychlorinated BiphenylsPopulationRecoveryReportingSamplingScientistScreening procedureSiteSoilStressSuperfundSystemSystems BiologyTechniquesTechnologyTimeToxicogenomicsUniversitiesUrsidae Familybasedechlorinationdibenzofurangenome sequencinghigh throughput screeninginsightinterestknowledge basemetabolic abnormality assessmentmicrobialmicrobial communitymolecular markerpollutantremediationresearch studystable isotopesuperfund chemicaltooltoxicant
项目摘要
The microbial world is diverse owing to its 3.7 billion years of evolution, which provides for both the
opportunity of undiscovered metabolic capacity, including that for pollutant degradation, and the challenge of
detecting and recovering this activity. It is well known that more than 99% of the microbial world has not
been cultured and hence remains undiscovered. We propose to explore and recover genes for two key
biodegradative steps in the detoxification of chlorinated polyaromatic compounds from the DNA of
this uncultured microbial diversity, and then to use the molecular markers from this study to aid in
site assessment and quantitative predictions of biodegradation at contaminated sites. We are
targeting the reductive dehalogenases and the aromatic oxygenases as the key functions to recover since
they are most often the rate limiting steps in the degradation of the polychlorinated dioxins and
dibenzofurans, PCBs and polynuclear aromatic hydrocarbons (PAHs), the Superfund chemicals of focus in
our study. Our specific aims are to: (1) explore and recover nature's catalytic diversity with the goal of
developing a comprehensive profile of microbial metabolic capabilities for these polyaromatic compounds,
(2) use the genome sequence information of Burkholderia xenovorans LB400, the most effective PCB
degrader, to study the metabolic features important to the degradation of these chemicals and (3), develop
quantitative diagnostic tools based on Bayesian probabilistic networks to predict biodegradation at
contaminated sites. We propose to use enrichments to help identify the functionally active populations and
hence genes, to use stable isotope probing to recover DNA from the active degrading populations, to use
metagenomic libraries to recover the full genes and operons, and to use high throughput PCR screening for
identifying clones with the targeted gene families. This project combines the expertise of the Rutgers
University scientists in aromatic oxygenases and high throughput screening and metagenomics with the
expertise at Michigan State University in reductive dechlorination and genomics and microarray technology.
微生物世界是多样化的,因为它有37亿年的进化历史,这为
未发现的代谢能力的机遇,包括污染物降解能力,以及
检测并恢复此活动。众所周知,超过99%的微生物世界没有
已被培养,因此仍未被发现。我们建议探索和恢复两个密钥的基因
细菌DNA中氯代多环芳香化合物脱毒的生物降解步骤
这种未经培养的微生物多样性,然后使用这项研究的分子标记来帮助
污染场地生物降解的场地评估和定量预测。我们是
以还原脱卤酶和芳香族加氧酶为关键功能恢复以来
它们通常是多氯二恶英降解的限速步骤和
二苯并呋喃、多氯联苯和多环芳烃(PAHs),超级基金关注的化学品
我们的书房。我们的具体目标是:(1)探索和恢复大自然的催化多样性,目标是
开发这些多芳香族化合物的微生物代谢能力的全面概况,
(2)利用最有效的印刷电路板异种伯克霍尔德氏菌LB400的基因组序列信息
降解剂,以研究对这些化学物质的降解重要的代谢特征和(3),开发
基于贝叶斯概率网络的生物降解性定量诊断工具
受污染的场地。我们建议使用富集物来帮助识别功能活跃的种群和
因此,使用稳定同位素探测从活跃的降解种群中恢复DNA的基因,以使用
元基因组文库回收完整的基因和操纵子,并使用高通量的PCR筛选
识别具有目标基因家族的克隆。这个项目结合了罗格斯大学的专业知识
大学科学家在芳香加氧酶和高通量筛选和元基因组学方面的研究
密歇根州立大学在还原脱氯、基因组学和微阵列技术方面的专业知识。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
JAMES M TIEDJE其他文献
JAMES M TIEDJE的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('JAMES M TIEDJE', 18)}}的其他基金
Molecular Insight into Polyaromatic Toxicant Degradation
多环芳烃有毒物质降解的分子洞察
- 批准号:
7064105 - 财政年份:2006
- 资助金额:
$ 43.56万 - 项目类别:
ECOLOGY AND DIVERSITY OF BTEX DEGRADING BACTERIA IN BIOREACTORS AND AQUIFERS
生物反应器和含水层中苯系物降解细菌的生态学和多样性
- 批准号:
6296553 - 财政年份:1999
- 资助金额:
$ 43.56万 - 项目类别:
ECOLOGY AND DIVERSITY OF BTEX DEGRADING BACTERIA IN BIOREACTORS AND AQUIFERS
生物反应器和含水层中苯系物降解细菌的生态学和多样性
- 批准号:
6106201 - 财政年份:1999
- 资助金额:
$ 43.56万 - 项目类别:
ECOLOGY AND DIVERSITY OF BTEX DEGRADING BACTERIA IN BIOREACTORS AND AQUIFERS
生物反应器和含水层中苯系物降解细菌的生态学和多样性
- 批准号:
6217616 - 财政年份:1999
- 资助金额:
$ 43.56万 - 项目类别:
ECOLOGY AND DIVERSITY OF BTEX DEGRADING BACTERIA IN BIOREACTORS AND AQUIFERS
生物反应器和含水层中苯系物降解细菌的生态学和多样性
- 批准号:
6271086 - 财政年份:1998
- 资助金额:
$ 43.56万 - 项目类别:
ECOLOGY AND DIVERSITY OF BTEX DEGRADING BACTERIA IN BIOREACTORS AND AQUIFERS
生物反应器和含水层中苯系物降解细菌的生态学和多样性
- 批准号:
6239503 - 财政年份:1997
- 资助金额:
$ 43.56万 - 项目类别:
Molecular Insight into Polyaromatic Toxicant Degradation by Microbial Communities
微生物群落对多环芳烃有毒物质降解的分子洞察
- 批准号:
7466403 - 财政年份:
- 资助金额:
$ 43.56万 - 项目类别:
ECOLOGY AND DIVERSITY OF BTEX DEGRADING BACTERIA IN BIOREACTORS AND AQUIFERS
生物反应器和含水层中苯系物降解细菌的生态学和多样性
- 批准号:
3733752 - 财政年份:
- 资助金额:
$ 43.56万 - 项目类别:
相似海外基金
Rational design of rapidly translatable, highly antigenic and novel recombinant immunogens to address deficiencies of current snakebite treatments
合理设计可快速翻译、高抗原性和新型重组免疫原,以解决当前蛇咬伤治疗的缺陷
- 批准号:
MR/S03398X/2 - 财政年份:2024
- 资助金额:
$ 43.56万 - 项目类别:
Fellowship
Re-thinking drug nanocrystals as highly loaded vectors to address key unmet therapeutic challenges
重新思考药物纳米晶体作为高负载载体以解决关键的未满足的治疗挑战
- 批准号:
EP/Y001486/1 - 财政年份:2024
- 资助金额:
$ 43.56万 - 项目类别:
Research Grant
CAREER: FEAST (Food Ecosystems And circularity for Sustainable Transformation) framework to address Hidden Hunger
职业:FEAST(食品生态系统和可持续转型循环)框架解决隐性饥饿
- 批准号:
2338423 - 财政年份:2024
- 资助金额:
$ 43.56万 - 项目类别:
Continuing Grant
Metrology to address ion suppression in multimodal mass spectrometry imaging with application in oncology
计量学解决多模态质谱成像中的离子抑制问题及其在肿瘤学中的应用
- 批准号:
MR/X03657X/1 - 财政年份:2024
- 资助金额:
$ 43.56万 - 项目类别:
Fellowship
CRII: SHF: A Novel Address Translation Architecture for Virtualized Clouds
CRII:SHF:一种用于虚拟化云的新型地址转换架构
- 批准号:
2348066 - 财政年份:2024
- 资助金额:
$ 43.56万 - 项目类别:
Standard Grant
BIORETS: Convergence Research Experiences for Teachers in Synthetic and Systems Biology to Address Challenges in Food, Health, Energy, and Environment
BIORETS:合成和系统生物学教师的融合研究经验,以应对食品、健康、能源和环境方面的挑战
- 批准号:
2341402 - 财政年份:2024
- 资助金额:
$ 43.56万 - 项目类别:
Standard Grant
The Abundance Project: Enhancing Cultural & Green Inclusion in Social Prescribing in Southwest London to Address Ethnic Inequalities in Mental Health
丰富项目:增强文化
- 批准号:
AH/Z505481/1 - 财政年份:2024
- 资助金额:
$ 43.56万 - 项目类别:
Research Grant
ERAMET - Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
ERAMET - 快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
- 批准号:
10107647 - 财政年份:2024
- 资助金额:
$ 43.56万 - 项目类别:
EU-Funded
Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
- 批准号:
10106221 - 财政年份:2024
- 资助金额:
$ 43.56万 - 项目类别:
EU-Funded
Recite: Building Research by Communities to Address Inequities through Expression
背诵:社区开展研究,通过表达解决不平等问题
- 批准号:
AH/Z505341/1 - 财政年份:2024
- 资助金额:
$ 43.56万 - 项目类别:
Research Grant