POLARIZED FCS

偏振FCS

• 批准号: 7724028
• 负责人: THEODORE HAZLETT
• 金额: $ 1.53万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7724028
• 关键词: Binding Proteins; Computer Retrieval of Information on Scientific Projects Database; Diffusion; Fluorescence; Funding; Grant; Institution; Light; Measures; Membrane; Motion; Movement; Research; Research Personnel; Resources; Rotation; Sampling; Series; Source; Spectrum Analysis; Techniques; Testing; Thinking; Time; United States National Institutes of Health; Vesicle; Work; fluorophore; particle; practical application; programs; simulation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Angular fluctuations measured through polarized fluorescence correlation spectroscopy. My work has focused on exploring the various avenues for measuring angular motion using FCS. Through simulations, using SimFCS and my own programs, I have tested and evaluated the use of polarized excitation light and measuring the polarized emission. Calculating the polarization time series from the polarized dual channel emission allows one to calculate the polarization autocorrelation curve that appears to follow the angular particle rotation independent of fluctuations due to translational diffusion. This separation is not complete when one uses polarized excitation light, as we once thought. In addition to simulations, samples with slow rotations, such as dsDNA fragments, fluorophores in small unilamellar vesicles, fluorophore movement in supported bilayers, and membrane-bound proteins are being examined to evaluate the practical application of the technique.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 通过偏振荧光相关光谱测量的角度波动。我的工作集中在探索各种途径测量角运动使用FCS。通过模拟，使用SimFCS和我自己的程序，我已经测试和评估了偏振激发光的使用和测量偏振发射。从偏振双通道发射计算偏振时间序列允许计算偏振自相关曲线，该曲线似乎遵循独立于由于平移扩散引起的波动的角粒子旋转。正如我们曾经认为的那样，当使用偏振激发光时，这种分离并不完全。除了模拟，样品与缓慢的旋转，如双链DNA片段，荧光团在小的单层囊泡，荧光团运动支持双层，膜结合蛋白质正在检查，以评估该技术的实际应用。