PROTEIN PROTEIN & PROTEIN LIGAND INTERACTIONS

抗荧光素抗体和部分蛋白质结构是 为了分离初级构象状态而进行检查 并估计涉及到的构象亚态的大小 抗体-半抗原相互作用。多种抗铁血红素的单抗 抗体将进行光谱检查，以确定 对不同的卟啉和血红素基团的亲和力。这些测量结果 将包括荧光偏振、稳态荧光和 CD-测量。使用不同的卟啉作为半抗原将允许我们 确定每个单抗的抗原群。

Antifluorescein antibodies and partial protein constructs are being examined in order to isolate the primary conformational states and estimate the magnitude of the conformational substates involved in antibody-hapten interactions. A variety of monoclonal anti-heme antibodies will be examined spectroscopically to characterize the affinity to different porphyrin and heme groups. These measurements will include fluorescence polarization, steady-state fluorescence and CD-measurements. Using different porphyrins as haptens will allow us to determine the antigenic group for each Mab.