PROTEIN PROTEIN & PROTEIN LIGAND INTERACTIONS

抗荧光素抗体和部分蛋白质构建体是 为了分离初级构象状态 并估计参与的构象亚态的大小， 抗体-半抗原相互作用。 多种抗血红素单克隆抗体 将通过光谱检查抗体以表征 对不同卟啉和血红素基团的亲和力。 这些测量 将包括荧光偏振、稳态荧光和 CD测量 使用不同的卟啉作为半抗原可以让我们 以确定每种Mab的抗原组。

Antifluorescein antibodies and partial protein constructs are being examined in order to isolate the primary conformational states and estimate the magnitude of the conformational substates involved in antibody-hapten interactions. A variety of monoclonal anti-heme antibodies will be examined spectroscopically to characterize the affinity to different porphyrin and heme groups. These measurements will include fluorescence polarization, steady-state fluorescence and CD-measurements. Using different porphyrins as haptens will allow us to determine the antigenic group for each Mab.