PROTEIN PROTEIN & PROTEIN LIGAND INTERACTIONS

蛋白质 蛋白质

• 批准号: 6206050
• 负责人: THEODORE HAZLETT
• 金额: $ 2.42万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6206050
• 关键词: biological products; biomedical resource; proteins

Antifluorescein antibodies and partial protein constructs are being examined in order to isolate the primary conformational states and estimate the magnitude of the conformational substates involved in antibody-hapten interactions. A variety of monoclonal anti-heme antibodies will be examined spectroscopically to characterize the affinity to different porphyrin and heme groups. These measurements will include fluorescence polarization, steady-state fluorescence and CD-measurements. Using different porphyrins as haptens will allow us to determine the antigenic group for each Mab.

抗荧光素抗体和部分蛋白质构建体是 为了分离初级构象状态 并估计参与的构象亚态的大小， 抗体-半抗原相互作用。 多种抗血红素单克隆抗体 将通过光谱检查抗体以表征 对不同卟啉和血红素基团的亲和力。 这些测量 将包括荧光偏振、稳态荧光和 CD测量 使用不同的卟啉作为半抗原可以让我们 以确定每种Mab的抗原组。