PHYTOCHROME SIGNAL TRANSDUCTION - THERMOSTABLE CYANOBACTERIAL PHYTOCHROMES

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 光敏色素包括植物中的红光/远红光传感器的主要家族。我们将尝试确定两个新的耐热蓝藻光敏色素（包括结构域的房屋发色团-藻蓝胆素）的结构。该构建体光转换并且仅为23.6kDa，并且我们还希望确定其在红光暴露时的Pr和Pfr形式。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Phytochromes comprise a principal family of red/far-red light sensors in plants. We will attempt structure determination of two new thermostable cyanobacterial phytochromes (comprising the domain which houses the chromophore - phycocyanobilin). This construct photoconverts and is only 23.6 kDa and we would like to also determine its Pr andf Pfr forms upon red light exposure.On the long run we would like to eventually determine homodimer structures of these domains and the complexes with PHY signaling domains.