PHYTOCHROME SIGNAL TRANSDUCTION - THERMOSTABLE CYANOBACTERIAL PHYTOCHROMES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 光敏色素是植物中一个主要的红光/远红光传感器家族。我们将尝试确定两个新的耐热蓝藻光敏色素的结构(包括含有发色团的结构域-藻蓝胆素)。这个结构可以光转化，只有23.6 kDa，我们还想确定它在红光照射下形成的Pr和f Pfr形式。从长远来看，我们希望最终确定这些结构域的同源二聚体结构以及与PHY信号域的复合体。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Phytochromes comprise a principal family of red/far-red light sensors in plants. We will attempt structure determination of two new thermostable cyanobacterial phytochromes (comprising the domain which houses the chromophore - phycocyanobilin). This construct photoconverts and is only 23.6 kDa and we would like to also determine its Pr andf Pfr forms upon red light exposure.On the long run we would like to eventually determine homodimer structures of these domains and the complexes with PHY signaling domains.