15N RELAXATION MEASUREMENTS ON COLD PROBE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在整个弛豫实验过程中，应采取特殊的预防措施，以保持温度恒定。样品加热的影响可能会产生一个显着的偏差，在提取的弛豫数据，并随后在分子运动的参数。首先，所提取的弛豫数据受到各个弛豫实验的数据点之间的温度变化（例如，以不同弛豫延迟记录的R2数据点）的影响。第二，不同类型的弛豫实验中的不同平均温度导致实验弛豫数据之间的不一致。几种实验方法成功地用于RT探针，以保持不同数据点和不同实验之间的温度恒定。然而，在冷探针的情况下，样品加热可能比RT探针大。我们实验的目的是比较RT和冷探针记录的实验数据，并试图纠正冷探针上的温度不稳定性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Special precautions should be taken to keep the temperature constant throughout relaxation experiments. The effect of sample heating may produce a significant bias in the extracted relaxation data and subsequently in parameters of molecular motions. First, the extracted relaxation data are affected by the temperature variations between data points of the individual relaxation experiments (e.g. R2 data points recorded with different relaxation delay). Second, a different mean temperature in different types of relaxation experiments results in inconsistency between experimental relaxation data. Several experimental approaches are successful used on RT probes to keep the temperature constant for different data points and between different experiments. & nbsp; However in case of cold probe the sample heating might be somehow larger comparing with RT probe. The goals of our experiments are to compare & nbsp; experimental data recorded on RT and cold probes, and attempt to correct temperature instability on a cold probe.