DETERMINATION OF FHA STRUCTURE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 PIP 3是一种重要的第二信使，参与信号转导和膜转运。在海马神经元中，PIP 3在轴突尖端的积聚启动轴突特化和神经元极性形成。GAKIN是一种驱动蛋白样运动蛋白，直接与PIP 3相互作用蛋白相互作用，并介导含PIP 3囊泡的转运。GAKIN中的结合位点定位于由FHA结构域编码的小区域。我们试图确定FHA结构域在其天然形式和复杂形式的三维结构，以揭示GAKIN和PIP 3之间的结合机制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. PIP3 is an important second messenger implicated in signal transduction and membrance transport. In hippocampal neurons, the accumulation of PIP3 athe tip of neurite initiastes the axon specification and neuronal polarity fomation. GAKIN, a kinesin-like motor protein, directly interats with a PIP3-interacting protein and mediates the transport of PIP3-Containing vesicles. The binding site in GAKIN was localized to a small region encoded by the FHA domain. We try to determine the 3D structure of FHA domain in its native form and complex form in order to reveal the binding mechanism between GAKIN and PIP3.