COPOLYMER-MEMBRANE PROJECT

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 泊洛沙姆是一种表面活性嵌段共聚物家族，其由在两端用亲水性聚（环氧乙烷）（PEO）部分封端的疏水性聚（环氧丙烷）（PPO）部分构成，已知通过阻止受损细胞的细胞内物质的泄漏来帮助密封被疾病或创伤损伤的透化细胞膜。尽管泊洛沙姆在恢复细胞膜完整性方面的重要性，但其潜在的密封机制尚不清楚。泊洛沙姆188（P188，分子量= 8400 g/mol，具有80wt%PEO）是其家族中第一个被测试为膜密封剂的，这是由于其在具有低毒性的药物中的广泛应用。此外，P188是一种有效的基因载体，通过内吞作用实现基因转移。P188和其他泊洛沙姆也已被包括在药物递送系统中，以通过延长其循环时间来空间稳定脂质体。泊洛沙姆药物设计的改进依赖于对泊洛沙姆和脂质膜之间相互作用的更好理解。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Poloxamer, a family of surface-active block copolymers constituted of a hydrophobic poly (propylene oxide) (PPO) moiety capped with hydrophilic poly (ethylene oxide) (PEO) moieties on the two ends, is known to help seal permeabilized cell membranes that are damaged by disease or trauma via arresting leakage of intracellular materials of the damaged cell. Despite poloxamer's importance in restoring cell membrane integrity, the underlying sealing mechanisms are not well understood. Poloxamer 188 (P188, molecular weight = 8400 g/mol, with 80 wt% PEO) was the first among its family tested to be a membrane sealant due to its wide application in pharmaceuticals with low toxicity. Besides, P188 is an efficient gene carrier with gene transfer accomplished via endocytosis. P188 and other poloxamers have also been included in drug delivery systems to sterically stabilize liposomes by prolonging their circulation time. Improvement on poloxamer design for pharmaceutical use relies on a better understanding of interactions between poloxamer and lipid membranes.