EVALUATION OF VASCULAR PERFUSION OF HUMANIZED MOUSE LIVER

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 磁共振显微镜-9T，含Gd造影剂 我们最初有一个简单的方法，包括简单的浸泡染色，看看血管是否会充盈。由于门脉和动脉两侧在肝细胞中都是死胡同，目前还不清楚我们是否有必要的血流路径。因此，我们将简单地开始。许多年前的一些早期结果给了我鼓舞。 人源化的小鼠肝脏在第一次肝素冲洗后用多聚甲醛灌流固定，并通过门静脉用血管扩张剂扩张。人类肝细胞约占小鼠肝脏的70%，但按肝叶分布并不均匀。如果我们能通过门静脉给固定的肝脏灌流 对比剂，那么核磁共振应该能告诉我们人性化区域的血管供应受到了多大的干扰。在某些结节区域，这在25-50u分辨率下应该很容易被观察到。我不知道我能不能找到胆总管，但逆行检查胆道系统可能也值得一试。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. MR microscopy - 9T, with gadolinium contrast agent We have an initial simple approach that will involve simple immersion staining to see if the vessels will fill. Since the portal and arterial sides are both dead ended in the hepatocytes, it is not clear that we would have the necessary flow path required. So we will start simply. Some earlier results many years ago give me encouragement. The humanized mouse livers were perfusion-fixed with paraformaldehyde after first heparin-flushing them and dilating them with a vasodilator via the portal vein. Human hepatocytes account for roughly 70% of the mouse liver but the distribution is not uniform by lobe. If we could perfuse the fixed liver through the portal vein with a contrast material then MRI should tell us how disrupted the vascular supply is to the humanized areas. In some nodular areas, this should be easy to visualize at 25-50u resolution. I don't know that I can find the common bile duct but a retrograde look at the biliary system might also be worth a try.