3-D ORGANIZATION OF GENE EXPRESSION

基因表达的 3D 组织

• 批准号: 7722404
• 负责人: DAVID L SPECTOR
• 金额: $ 1.56万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722404
• 关键词: 3-Dimensional; Chromatin Structure; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Electron Microscope; Funding; Gene Expression; Genetic Transcription; Goals; Grant; Image; Institution; Life; Morphology; Nucleoplasm; Research; Research Personnel; Resources; Site; Source; System; Techniques; Transcriptional Activation; United States National Institutes of Health; messenger ribonucleoprotein; tomography

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our goal is to investigate the chromatin structure during activation of transcription and to examine mRNP morphology at the transcription site and in the nucleoplasm using tetracysteine-biarsenical probes, correlated live imaging, and electron microscope tomography. The application and adaptation of these techniques to our system will be developed in collaboration with NCMIR/UCSD.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们的目标是研究转录激活过程中的染色质结构，并使用四半胱氨酸-二砷探针、相关的实时成像和电子显微镜断层扫描来检查转录部位和核质中mRNP的形态。这些技术在我们的系统中的应用和改编将与NCMIR/UCSD合作开发。