GENE EXPRESSION USING ELECTRON MICROSCOPY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 微阵列提供了绝大多数基因的多个RNA异构体的加权平均值，建立起来既昂贵又繁琐，而且对极少量的RNA容易出错。我们建议开发一种单分子方法来进行表达谱分析，其中每个mRNA分子都使用部分简并的LNA进行标记，胶体金粒子连接到这些LNA上。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Microarrays provide a weighted average from multiple RNA isoforms for the vast majority of genes, are expensive and cumbersome to set up, and are error-prone for very small amounts of RNA. We propose to develop a single molecule approach to expression profiling, where each mRNA molecule is tagged using partially degenerate LNAs to which colloidal gold particles are attached.