GENE EXPRESSION USING ELECTRON MICROSCOPY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 研究中心，而研究中心不一定是研究者所在的机构。 微阵列提供了绝大多数基因的多种RNA亚型的加权平均值，设置昂贵且繁琐，并且对于非常少量的RNA容易出错。我们建议开发一种单分子的方法来表达谱，其中每个mRNA分子使用部分简并的LNA标记，其中胶体金颗粒附着。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Microarrays provide a weighted average from multiple RNA isoforms for the vast majority of genes, are expensive and cumbersome to set up, and are error-prone for very small amounts of RNA. We propose to develop a single molecule approach to expression profiling, where each mRNA molecule is tagged using partially degenerate LNAs to which colloidal gold particles are attached.