STRUCTURAL STUDIES OF ROTAVIRUS POLYMERASE VP1

轮状病毒聚合酶VP1的结构研究

• 批准号: 7721237
• 负责人: STEPHEN COPLAN HARRISON
• 金额: $ 4.94万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-15 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721237
• 关键词: Acute; Biological Models; Capsid; Cells; Child; Computer Retrieval of Information on Scientific Projects Database; Coupling; Double-Stranded RNA; Event; Funding; Gastroenteritis; Genetic Transcription; Genome; Grant; In Vitro; Institution; Polymerase; Proteins; RNA; Research; Research Personnel; Resources; Rotavirus; Source; United States National Institutes of Health; pathogen; viral RNA

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Rotaviruses, which cause acute gastroenteritis in children, have been extensively studied both as model systems and as pathogens. The transcription ((+)-strand synthesis from dsRNA) and replication ((-)-strand synthesis from (+)-strand ssRNA) of viral RNAs are both carried out by rotavirus polymerase VP1. in vitro replication of (-)-strand RNA requires the presence of the core lattice protein VP2. Determining the events leading to the synthesis of the (-)-strand viral RNA is essential to understanding the replication of rotavirus in host cells in general, and the coupling of genome replication and capsid assembly in particular.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 轮状病毒可引起儿童急性胃肠炎，作为模型系统和病原体已被广泛研究。病毒RNA的转录(+)链合成和复制(-)链合成都是由轮状病毒聚合酶VP1完成的。在体外复制(-)链RNA需要核心晶格蛋白VP2的存在。确定导致(-)链病毒RNA合成的事件对于了解轮状病毒在宿主细胞中的复制，特别是基因组复制和衣壳组装的耦合是至关重要的。