PHENOTYPING SCIENCE

表型科学

• 批准号: 7535429
• 负责人: Derry Charles Roopenian
• 金额: $ 43.28万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7535429
• 关键词: Arts; Biological Assay; Cancer Center; Cancer Center Support Grant; Cellular biology; Color; Computational Science; Ensure; Faculty; Fee-for-Service Plans; Flow Cytometry; Gene Expression; Gene Expression Microarray Analysis; Gene Targeting; Grant; Health Services Accessibility; Laboratories; Laboratory Research; Methods; Microchemistry; Microinjections; Molecular; Molecular Biology; Monoclonal Antibodies; Nucleic Acids; Operative Surgical Procedures; Phenotype; Protein Chemistry; Proteins; Research; Research Personnel; Resources; SNP genotyping; Science; Scientist; Services; Sorting - Cell Movement; Techniques; Technology; Transgenic Organisms; anticancer research; cost; design and construction; improved; member; reproductive; tool

Phenotyping Sciences provides Cancer Center faculty with diverse tools, techniques and expertise for the conduct of gene expression, molecular biology, flow cytometry, and protein chemistry methods. Created as a result of the reorganization of Scientific Services in 2004, this department comprises the former Microchemistry and Flow Cytometry Services. Microchemistry has been supported by the Cancer Center Support Grant since the Service was developed 17 years ago, while Flow Cytometry has been supported for the full 23-year term of the grant. The Phenotyping Sciences Service provides researchers with access to state-of-the-art gene expression technologies, nucleic acid isolation and quality assessment, gene targeting and transgenic construct design, protein chemistry services, advanced flow cytometric technology and a monoclonal antibody resource. Access to these services is essential to Cancer Center staff. Overall use of the Phenotyping Sciences Service has increased by 55% over the past five years. The Phenotyping Sciences Project Leader, Senior Staff Scientist Dr. Derry Roopenian, oversees this fee-for-service operation. Three gene expression technologists, two flow cytometrists, three molecular biologists, a protein chemist, and senior manager staff the facility which occupies 1,851 ft2 of laboratory space in the Research Laboratory Unit 4 building. The Service is dynamic, continuously increasing and improving its array of services to meet the needs of the Cancer Center members. Since the last renewal, significant advancements in technology have provided for the expansion and improvement of multi-color flow cytometry and sorting, gene expression microarray analysis, and multiplexed protein assay services. Phenotyping Sciences is completely integrated with the other services at the Center, including Computational Sciences, Reproductive Sciences-Cell Biology and Microinjection, and SNP Genotyping to provide a comprehensive set of support services for cancer research. Dr. Roopenian communicates with the Cancer Center users, Service staff and Center Administration to ensure that research needs are met in the most efficient, cost-effective and technically current manner.

表型科学为癌症中心的教职员工提供了多种工具、技术和专业知识， 进行基因表达、分子生物学、流式细胞术和蛋白质化学方法。创建为 由于2004年科学服务的重组，该部门包括前 微量化学和流式细胞术服务。微量化学得到了癌症中心的支持 自17年前开发该服务以来，一直获得支持补助金，同时流式细胞术也得到了支持 在23年的补助期内。表型科学服务为研究人员提供了 最先进的基因表达技术，核酸分离和质量评估，基因 靶向和转基因构建体设计，蛋白质化学服务，先进的流式细胞术技术 和单克隆抗体资源。获得这些服务对癌症中心的工作人员至关重要。整体 表型科学服务的使用在过去五年中增加了55%。的表型分型 科学项目负责人，高级科学家德里Roopenian博士，监督这项收费服务 操作三个基因表达技术专家，两个流式细胞仪，三个分子生物学家，一个蛋白质 化学家和高级管理人员的设施，占用1,851平方英尺的实验室空间的研究 实验室4号楼。该处是动态的，不断增加和改进其一系列 以满足癌症中心成员的需求。自上次更新以来， 技术的进步为多色流的扩展和改进提供了条件 细胞计数和分选、基因表达微阵列分析和多重蛋白质测定服务。 表型科学与中心的其他服务完全集成，包括 计算科学，生殖科学-细胞生物学和显微注射，以及SNP基因分型， 为癌症研究提供全面的支援服务。Roopenian博士与 癌症中心用户，服务人员和中心管理，以确保研究需求得到满足， 最有效、最具成本效益和技术上最先进的方式。