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BECTON-DICKINSON FACSCALIBUR FLOW CYTOMETRY SYSTEM

BECTON-DICKINSON FACSCALIBUR 流式细胞术系统

基本信息

批准号：
6054046
负责人：
Derry Charles Roopenian
金额：
$ 15.09万
依托单位：
JACKSON LABORATORY
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
起止时间：
2000-04-01 至 2001-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6054046
关键词：
biomedical equipment purchase flow cytometry

项目摘要

New genetic technologies, combined with a well-characterized genome, make the mouse the preferred mammalian model for human disease. The generation and characterization of mouse strains harboring genetically predetermined pathobiological syndromes is critical to biomedical research, and researchers at the Jackson Laboratory (TJL) are at the forefront of this endeavor. Full exploitation of mouse models rely on access to critical technologies. Over 35% of TJL's research staff rely heavily on flow cytometric techniques to carry out studies in the areas of immunology, diabetes, hemopoietic stem cell biology, gene therapy and cancer research. Flow cytometry constitutes an essential tool for quantitative multiparameter measurements of membrane and internal proteins on individual cells, for gene expression analysis and cell cycle regulation. TJL's current flow cytometry instrumentation is overburdened and it is becoming too outmoded to meet the demands of our NIH funded research projects. The basic data collection limitations make it more costly to carryout multiparameter analyses and thus limit the application of emerging flow technologies necessary to remain competitive. The Becton-Dickinson FACSCalibur Flow Cytometry System has proven its reliability in both clinical and research settings. Because of its automated sample loader, flexible and efficient sorting capability and user friendly interface, the FACSCalibur is a cost-efficient solution enabling investigators to maximize the potential of their mouse models. Funds are requested to purchase the FACSCalibur Flow Cytometry System, to be operated by the institution's Flow Cytometry Service thus providing a cost-effective way to fill an urgent and unmet need of TJL Staff.

新的基因技术，加上特征明确的基因组，使小鼠成为人类疾病的首选哺乳动物模型。携带遗传预定病理生物学综合征的小鼠品系的产生和特征对于生物医学研究至关重要，杰克逊实验室(TJL)的研究人员处于这一努力的前沿。对鼠标模型的充分开发依赖于关键技术的获取。超过35%的TJL研究人员严重依赖流式细胞仪技术来开展免疫学、糖尿病、造血干细胞生物学、基因治疗和癌症研究等领域的研究。流式细胞术是对单个细胞的细胞膜和内部蛋白质进行多参数定量测量、基因表达分析和细胞周期调控的重要工具。TJL目前的流式细胞仪负担过重，而且太过时了，无法满足我们NIH资助的研究项目的需求。基本的数据收集限制使进行多参数分析的成本更高，从而限制了保持竞争力所必需的新兴流动技术的应用。Becton-Dickinson FACSCalibur流式细胞术系统已在临床和研究环境中证明了其可靠性。由于其自动样品加载器、灵活高效的分类能力和用户友好的界面，FACSCalibur是一种经济高效的解决方案，使研究人员能够最大限度地发挥他们的鼠标模型的潜力。需要资金购买FACSCalibur流式细胞仪系统，该系统将由该机构的流式细胞仪服务处操作，从而提供一种具有成本效益的方式来满足TJL工作人员的紧急和未得到满足的需求。