DNA ANALYSIS/AUTO SEQUENCING AND PHOSPHORIMAGING

DNA 分析共享资源 托马斯·泰德博士，董事 杜克综合癌症中心 DNA 分析共享资源由两个相关设施组成， 磷光成像设备和 DNA 测序设备。该资源服务于跨学科 来自癌症中心、医学中心和大学的研究人员小组。这些调查员来自 基础科学和临床科学领域具有广泛的兴趣，包括癌症生物学、基础科学 分子生物学、信号转导、发育机制、细胞对感染和人类的反应 癌症、免疫缺陷、关节炎和心脏病等疾病。 Phosphofimager 设施允许 研究人员使用放射自显影、荧光和 化学荧光检测。这项技术使科学家能够轻松地进行准确的信号检测 定量上超过几个数量级，同时减少了曝光时间。 DNA测序 设施提供自动化 DNA 测序、DNA 片段分析和突变检测，以实现快速 基因结构和功能分析。这两个设施结合起来提供了一种综合方法 满足杜克大学社区的 DNA 分析需求。此外，还加快了研究速度 通过替换旧的、低效且昂贵的手动操作，显着节省数据生成的时间 具有最先进的自动化程序的方法。该资源提供了基于荧光的新方法 减少我们对放射性使用的依赖的技术。

DNA ANALYSIS SHARED RESOURCE Thomas Tedder, Ph.D., Director The Duke Comprehensive Cancer Center DNA Analysis Shared Resource consists of two related facilities, the Phosphorimager Facility and the DNA Sequencing Facility. This resource serves an interdisciplinary group of investigators from the Cancer Center, Medical Center and University. These investigators come from both the basic and clinical science areas with a wide range of interests including cancer biology, basic molecular biology, signal transduction, mechanisms of development, cellular response to infection and human diseases such as cancer, immunodeficiency, arthritis and heart disease. The Phosphofimager Facility allows researchers to analyze a wide variety of DNA and protein samples using autoradiography, fluorescence, and chemifluorescence detection. This technology permits scientists to easily perform accurate signal detection quantitatively over several orders of magnitude along with reduced exposure times. The DNA Sequencing Facility provides automated DNA Sequencing, DNA fragment analysis and mutation detection for rapid analysis of gene structure and function. Together, these two facilities combine to offer an integrated approach to serving the DNA analysis needs of the Duke University community. In addition, research is expedited by providing significant time savings in the production of data by replacing older, inefficient, and costly manual methods with state-of-the art, automated procedures. This resource makes available new fluorescence based techniques that reduce our dependence on the use of radioactivity.