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DNA ANALYSIS/AUTO SEQUENCING AND PHOSPHORIMAGING

DNA 分析/自动测序和磷成像

基本信息

批准号：
7764702
负责人：
THOMAS F TEDDER
金额：
$ 17.45万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7764702
关键词：
Academic Medical Centers Area Arthritis Arts Autoradiography Basic Science Biological Assay Cancer Biology Cancer Center Cellular Immunity Chromosome Mapping Clinical Investigator Clinical Sciences Communities DNA DNA Sequence DNA Sequencing Facility DNA analysis Data Dependence Detection Development Doctor of Philosophy Duke Comprehensive Cancer Center Fluorescence Gene Expression Gene Structure Genotype Goals Grant Heart Diseases Immunodeficiency and Cancer Infection Laboratories Malignant Neoplasms Manuals Methods Molecular Biology Mutation Mutation Detection Procedures Production Proteins Radioactivity Research Research Personnel Research Project Grants Resource Sharing Resources Sampling Savings Scientist Screening procedure Services Signal Transduction Techniques Technology Time Universities base cellular development human disease interest response

项目摘要

DNA ANALYSIS SHARED RESOURCE Thomas Tedder, Ph.D., Director The Duke Comprehensive Cancer Center DNA Analysis Shared Resource consists of two related facilities, the Phosphorimager Facility and the DNA Sequencing Facility. This resource serves an interdisciplinary group of investigators from the Cancer Center, Medical Center and University. These investigators come from both the basic and clinical science areas with a wide range of interests including cancer biology, basic molecular biology, signal transduction, mechanisms of development, cellular response to infection and human diseases such as cancer, immunodeficiency, arthritis and heart disease. The Phosphofimager Facility allows researchers to analyze a wide variety of DNA and protein samples using autoradiography, fluorescence, and chemifluorescence detection. This technology permits scientists to easily perform accurate signal detection quantitatively over several orders of magnitude along with reduced exposure times. The DNA Sequencing Facility provides automated DNA Sequencing, DNA fragment analysis and mutation detection for rapid analysis of gene structure and function. Together, these two facilities combine to offer an integrated approach to serving the DNA analysis needs of the Duke University community. In addition, research is expedited by providing significant time savings in the production of data by replacing older, inefficient, and costly manual methods with state-of-the art, automated procedures. This resource makes available new fluorescence based techniques that reduce our dependence on the use of radioactivity.

DNA分析共享资源托马斯特德博士，主任杜克综合癌症中心DNA分析共享资源由两个相关设施组成，磷光成像仪和DNA测序仪。该资源提供跨学科的来自癌症中心、医学中心和大学的一组研究人员。这些调查人员来自基础和临床科学领域，兴趣广泛，包括癌症生物学，基础分子生物学，信号转导，发育机制，细胞对感染的反应和人类癌症、免疫缺陷、关节炎和心脏病等疾病。Phosphofimager设施允许研究人员利用放射自显影、荧光和化学荧光检测这项技术使科学家能够轻松地进行准确的信号检测随着曝光时间的减少，在数量上可以达到沿着几个数量级。DNA测序设施提供自动化DNA测序，DNA片段分析和突变检测，基因结构和功能分析。这两种设备联合收割机共同提供了一种集成方法以满足杜克大学社区的DNA分析需求。此外，研究还加快了通过替换旧的、低效的和昂贵的手工操作，最先进的自动化程序。该资源使得新的荧光基减少我们对放射性的依赖的技术。