Ordered Arrays for Advanced Sequencing Systems

用于高级测序系统的有序阵列

• 批准号: 7980185
• 负责人: Steven Jeffrey Gordon
• 金额: $ 122.11万
• 依托单位: INTELLIGENT BIO-SYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-19 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7980185
• 关键词: Agreement; Applications Grants; Biochemical Reaction; Biology; Chemicals; Chemistry; Communities; Complex; DNA; DNA Sequence; Data; Detection; Diagnostic; Escherichia coli; Evaluation; Generations; Genome; Genotype; Goals; Government; Grant; Image; Individual; Label; Lead; Length; Libraries; Licensing; Measures; Medicine; Methods; New England; Noise; Nucleotides; Optics; Organism; Output; Persons; Pharmacogenomics; Phase; Polymerase; Preparation; Reading; Reporting; Research; Research Personnel; Running; Sampling; Signal Transduction; Small Business Innovation Research Grant; Speed; Spottings; Symptoms; Synthesis Chemistry; System; Techniques; Technology; Therapeutic; Time; Universities; Variant; base; cost; cost effective; density; design; innovation; instrument; next generation; novel; nucleotide analog; prototype; public health relevance; single molecule; tool

DESCRIPTION (provided by applicant): The advent of next-generation sequencing technologies is allowing researchers to perform studies and make discoveries which previously were not economically or technically feasible. Thus far, however, higher-throughput next-generation sequencing systems are relatively expensive, have relatively long run times and produce relatively short reads thereby limiting their use for diagnostic applications. In this Phase II application, we propose to combine the novel chip fabrication techniques developed in Phase I, the innovative sequencing by synthesis chemistry exclusively licensed from Columbia University, and an automated prototype sequencing instrument to produce an advanced sequencing by synthesis system. This system will be higher throughput and significantly more cost effective than other competing next-generation technologies. During the project, high density chips will be fabricated, the sequencing instrument and chemistry will be optimized and an E. coli genome will be re-sequenced. This system will be capable of producing large amounts of quality sequence data faster and at a lower cost than any other near-term next generation sequencing system. This will make next-generation DNA sequencing technology more accessible to the broad research community. PUBLIC HEALTH RELEVANCE: Ultimately, the ability to produce very inexpensive detailed DNA sequence information for complex organisms' genomes will both lead to accelerated discoveries throughout biology and provide the basis for Pharmacogenomics, a new paradigm in therapeutics wherein medicines are prescribed based on individual genotypes rather than just observed symptoms.

描述（由申请人提供）：下一代测序技术的出现使研究人员能够进行研究并发现以前在经济上或技术上不可行的发现。 然而，到目前为止，更高通量的下一代测序系统相对昂贵，具有相对长的运行时间并且产生相对短的读取，从而限制了它们用于诊断应用。 在第二阶段的应用中，我们建议将第一阶段开发的新型芯片制造技术、哥伦比亚大学独家授权的创新的合成化学测序和自动化原型测序仪器相结合，以产生先进的合成测序系统。该系统将比其他竞争的下一代技术具有更高的吞吐量和更高的成本效益。 在该项目中，将制造高密度芯片，优化测序仪器和化学，并在2010年推出E。大肠杆菌基因组将被重新测序。 该系统将能够比任何其他近期的下一代测序系统更快、更低成本地产生大量高质量的序列数据。 这将使下一代DNA测序技术更容易为广大研究界所接受。 公共卫生关系：最终，为复杂生物体的基因组产生非常便宜的详细DNA序列信息的能力将加速整个生物学的发现，并为药物基因组学提供基础，药物基因组学是一种新的治疗模式，其中药物是基于个体基因型而不仅仅是观察到的症状。