Streamlined Template Preparation for Advanced Sequencing Methods

描述（由申请人提供）：下一代测序技术的出现使研究人员能够进行以前在经济或技术上不可行的研究和发现。例如，对肿瘤组织中基因组序列的精确了解可以作为治疗的指标（类似于乳腺癌的erbB-2受体基因Her-2）。尽管在产生序列数据的核心化学物质和仪器方面进行了大量的研究和开发工作，但很少有新技术应用于制备高通量或临床使用的样品。特别是，使用珠子作为扩增和帮助定位测序芯片上样品的坚实支持的测序系统仍然需要繁琐的手工处理。随着实验室扩大使用新的测序技术，他们很快意识到操作中的瓶颈是样品制备。在第二期申请中，我们建议采取远远超出第一期图书馆建设范围的样品制备步骤。我们建议自动化整个样品制备过程，从DNA片段后开始，通过文库构建，直到附着到芯片之前结束。拟议的系统将与一些正在开始进入市场的下一代系统兼容。

DESCRIPTION (provided by applicant): The advent of next-generation sequencing technologies is allowing researchers to perform studies and make discoveries which previously were not economically or technically feasible. For example, precise knowledge of the genomic sequence within neoplastic tissues can be an indicator for treatment (similar to the erbB-2 receptor gene, Her-2, for breast cancer). Although much research and development effort has gone into the core chemistries and instruments which actually produce sequence data, few new techniques have been applied to the preparation of samples for high-throughput or use in the clinic. In particular, sequencing systems that use beads as both the solid support for amplification and to help localize samples on a sequencing chip still require tedious processing by hand. As labs scale up to use the new sequencing technologies, they are very quickly realizing that the bottleneck in the operation is in the sample preparation. In this Phase II application, we propose to take the sample preparation steps far beyond the original Phase I scope of library construction. We propose to automate the entire sample preparation process, starting just after DNA fragmentation, through library construction and ending just prior to attachment to a chip. The proposed system will be compatible with a number of next generation systems which are starting to enter the market.