Streamlined Template Preparation for Advanced Sequencing Methods

简化高级测序方法的模板准备

• 批准号: 7622607
• 负责人: Steven Jeffrey Gordon
• 金额: $ 75.98万
• 依托单位: INTELLIGENT BIO-SYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-09 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7622607
• 关键词: Biology; Chemistry; Clinic; Complex; DNA; DNA Fragmentation; DNA Sequence; Data; Deposition; Emulsions; Genome; Genomics; Genotype; Goals; Hand; Individual; Knowledge; Laboratories; Lead; Libraries; Magnetism; Manuals; Medicine; Methods; Operative Surgical Procedures; Organism; Pharmacogenomics; Phase; Preparation; Process; Protocols documentation; Receptor Gene; Reporting; Research; Research Personnel; Robot; Robotics; Sampling; Small Business Innovation Research Grant; Solid; Symptoms; System; Techniques; Technology; Therapeutic; Tissues; Work; base; density; design; erbB-2 Receptor; innovation; instrument; magnetic beads; malignant breast neoplasm; neoplastic; next generation; prototype; research and development; scale up

DESCRIPTION (provided by applicant): The advent of next-generation sequencing technologies is allowing researchers to perform studies and make discoveries which previously were not economically or technically feasible. For example, precise knowledge of the genomic sequence within neoplastic tissues can be an indicator for treatment (similar to the erbB-2 receptor gene, Her-2, for breast cancer). Although much research and development effort has gone into the core chemistries and instruments which actually produce sequence data, few new techniques have been applied to the preparation of samples for high-throughput or use in the clinic. In particular, sequencing systems that use beads as both the solid support for amplification and to help localize samples on a sequencing chip still require tedious processing by hand. As labs scale up to use the new sequencing technologies, they are very quickly realizing that the bottleneck in the operation is in the sample preparation. In this Phase II application, we propose to take the sample preparation steps far beyond the original Phase I scope of library construction. We propose to automate the entire sample preparation process, starting just after DNA fragmentation, through library construction and ending just prior to attachment to a chip. The proposed system will be compatible with a number of next generation systems which are starting to enter the market.

描述（由申请人提供）：下一代测序技术的出现使研究人员能够进行研究并发现以前在经济上或技术上不可行的发现。例如，肿瘤组织内基因组序列的精确知识可以作为治疗的指标（类似于乳腺癌的erbB-2受体基因Her-2）。虽然许多研究和开发工作已经进入核心化学和仪器，实际上产生序列数据，很少有新技术已被应用于高通量或在临床中使用的样品的制备。特别地，使用珠作为扩增的固体支持物并帮助将样品定位在测序芯片上的测序系统仍然需要手工进行繁琐的处理。随着实验室扩大规模使用新的测序技术，他们很快意识到操作的瓶颈在于样品制备。在第二阶段申请中，我们建议采取的样本制备步骤远远超出了最初的第一阶段库建设范围。我们建议将整个样品制备过程自动化，从DNA片段化后开始，通过文库构建，并在连接到芯片之前结束。拟议的系统将与一些开始进入市场的下一代系统兼容。