Development of a Hyper-spectral Spectroscopic Instrument for Non-invasive Diagnos

描述（由申请人提供）：对疾病的非侵入性和早期识别存在迫切需求。使用光谱仪器的“光学活检”提供了一种立即确定组织病理学的方法，而不需要手术活检。我们提出了两种光谱系统的非侵入性检测和边缘划界：1）深度敏感的光纤探头（目标1）的超光谱光谱系统（目标2）。我们将开发一种深度敏感光纤探头来测量深度相关的光学特征。我们将开发一个光谱系统，将基于探针的光学活检转换为光谱格式。这些仪器系统可以协同使用，以利用高光谱光谱设备来测量广泛的组织区域，然后利用探针提供详细的深度敏感分析。我们将使用皮肤癌作为模型系统来测试新的光谱仪器。例如，癌症的早期识别可以提高患者的生存率，而非侵入性和快速识别癌症将降低发病率。特别重要的是恶性肿瘤的深度，其通常用于疾病分期（例如原位癌与浸润性癌）。我们将在临床初步研究中确定每种器械与标准组织病理学相比的灵敏度和特异性（目标3）。 公共卫生相关性：迫切需要开发微创便携式技术。在过去的十年中，光学光谱学已被开发用于评估组织病理学，并已被证明可以提供详细的形态学和生化信息，而无需去除组织。该定量信息可用于进行最终诊断（所谓的“光谱诊断”），类似于病理学家使用组织学。光谱诊断（SD）是非侵入性的，不需要对组织进行活检、固定、处理或显微镜检查。拟议的项目代表了一个跨学科的努力，以开发技术的非侵入性检测皮肤癌。这些技术提供了降低与皮肤恶性肿瘤相关的发病率和死亡率的可能性。

DESCRIPTION (provided by applicant): A critical need exists for the non-invasive and early identification of diseases. "Optical biopsy" using spectroscopic instruments offers a means to determine tissue pathology immediately and without the need for surgical biopsy. We propose to develop two spectroscopic systems for noninvasive detection and margin demarcation: 1) a depth sensitive fiber probe (Aim 1) a hyper spectral spectroscopic system (Aim 2). We will develop a depth-sensitive optical fiber probe to measure depth-dependent optical signatures. We will develop a spectroscopic system to translate probe based optical biopsy to a spectral format. These instrument systems can be used in concert to survey wide areas of tissue with the hyper-spectral spectroscopic device and then provide a detailed depth-sensitive analysis with the probe. We will use skin cancer as a model system to test the new spectroscopic instrument. For example, early identification of cancer increases patient survival while non-invasive and rapid identification of cancer will decrease morbidity. Of particular importance is the depth of a malignancy which is often used to stage disease (e.g. carcinoma in situ vs. invasive carcinoma). We will determine the sensitivity and specificity of each device compared to standard histopathology in a clinical pilot study (Aim 3). PUBLIC HEALTH RELEVANCE: There is a critical need for the development of less invasive portable technologies. Over the past decade, optical spectroscopy has been developed to assess tissue pathology and has been shown to provide detailed morphological and biochemical information without tissue removal. This quantitative information can be used to make a final diagnosis (a so called "spectral diagnosis") similar to a pathologist using histology. A spectral diagnosis (SD) is non- invasive, does not require biopsy, fixation, processing or microscopic examination of tissue. The proposed project represents an interdisciplinary effort to develop technologies for the non- invasive detection of skin cancers. Such technologies offer the possibility to reduce morbidity and mortality associated with skin malignancy.