The Development of Immune-based Therapies Using Plant Derived Compounds to Attenu

描述（由申请方提供）：将从特定植物材料中提取次级代谢产物，以鉴定和分离免疫活性成分。这些组分被认为在人类免疫缺陷病毒（HIV）发展为获得性免疫缺陷综合征（AIDS）之前通过调节白色血细胞中的某些细胞因子水平来增强先天性和适应性免疫系统。鉴定出的成分将用于开发新的抗HIV免疫疗法。已经鉴定了含有具有所需免疫活性特征的组分的植物材料。建立了从植物中提取次生代谢产物（非聚合小分子）的方法和检测白色血细胞中人细胞因子的抗体法。该项目的第一阶段包括使用反相高效液相色谱（HPLC）、离子交换色谱（IEC）、核磁共振（NMR）和质谱（MS）从植物提取物中鉴定和分离免疫活性成分。将确定植物中活性成分的百分比。该项目的第二阶段将需要大规模分离/纯化或合成已鉴定的免疫活性植物成分，以开发体外和体内新的抗艾滋病毒免疫疗法的筛选。所提出的疗法可以减少病毒载量，间接降低艾滋病毒对目前抗病毒药物的耐药性。 公共卫生相关性：该项目旨在从特定植物中分离免疫活性成分，这些成分可以调节人类白色血细胞中的某些细胞因子水平，从而提供基于免疫的治疗活性，以减缓艾滋病毒的进展。该项目的最终结果将具有作为一线治疗和协同化合物的巨大商业潜力，可以间接降低艾滋病毒对当前抗病毒药物的耐药性。我们相信，我们的潜在产品将单独使用，作为新诊断患者的初始治疗，或与目前的抗病毒药物联合使用。

DESCRIPTION (provided by applicant): Secondary metabolites will be extracted from a specific plant material to identify and isolate immune active components. These components are proposed to enhance the innate and adaptive immune systems by modulating certain cytokine levels in white blood cells before the progression of Human Immunodeficiency Virus (HIV) to Acquired Immune Deficiency Syndrome (AIDS) occurs. The components identified will be used to develop new anti-HIV immune-based therapies. The plant material containing the components with desirable immune active characteristics has been identified. The methods suitable for extraction of secondary metabolites (non-polymeric small molecules) from the plant material and antibody-based assay for testing of human cytokines in white blood cells have been established. Phase I of this project includes identification and isolation of immune active components from the plant extract using reverse phase High Performance Liquid Chromatography (HPLC), Ion-Exchange Chromatography (IEC), Nuclear Magnetic Resonance (NMR) and Mass Spectrometry (MS). The percentage of active components in the plant will be determined. Phase II of the project will entail either large scale isolation/purification or synthesis of identified immune active plant components to develop screens for new anti-HIV immune-based therapies both in vitro and in vivo. The therapies proposed can reduce viral load and indirectly lower the resistance of HIV to current antiviral drugs. PUBLIC HEALTH RELEVANCE: The project seeks to isolate immune active components from specific plants that can modulate certain cytokine levels in human white blood cells which would provide an immune-based therapeutic activity to attenuate HIV progression. The end result of this project would have substantial commercial potential as a frontline therapy and synergistic compound that can indirectly lower the resistance of HIV to current antiviral drugs. We believe that our potential product will be used either alone, as an initial therapy for newly diagnosed patients, or in combination with current antiviral drugs.