Plug and Play Bioreactor

描述（由申请人提供）：我们建议开发一种新的生物反应器系统，该系统将从根本上推进人类细胞和工程组织的生物医学和临床研究。该项目将解决长期以来对培养系统的需求，该培养系统提供高生物保真度沿着精确的环境控制、真实的时间成像和自适应操作策略，其配置易于被广泛的研究者使用。目前可用的生物反应器系统的使用大大提高了我们进行基础和转化研究的能力。然而，主要的限制仍然存在。需要将联合收割机结合几个关键要求的生物反应器：（i）提供类似于体内遇到的那些的精确控制的3D环境，（ii）应用多种调节因子（分子、物理、细胞和基质衍生的），（iii）用于高通量和组合研究的模块化设计，以及（iv）用于实时洞察的实时成像兼容性。我们的实验室已经开发了近二十年的组织工程生物反应器。我们建议整合和推进我们积累的经验和资源，以开发真正先进的新型生物反应器，与生物医学和临床研究具有广泛的相关性。三个具体的目标将追求在一个综合的方式，以优化和全面验证拟议的生物反应器系统。目的1是开发一种台式生物反应器平台，其培养模块由具有介质流量和环境控制的自维持盒组成。目的2是开发具有介质灌注和机械加载的生物反应器配置，适用于习惯性加载细胞和组织的研究。目的三是研制一种具有灌注、电拉伸和机械拉伸功能的生物反应器，适用于机电活性细胞和组织的研究。拟议的系统将以一个共同平台为基础，该平台提供一套基本功能，将与在线成像、环境控制和数据采集接口。我们工作的重点将是干细胞和组织工程研究的生物学要求，以及生物反应器设计的全新工程解决方案的实施。 公共卫生相关性（由申请人提供）：生物反应器的开发将使人类细胞和工程组织的研究在类似于体内的条件下，精确的环境控制和实时成像将真正推动生物和医学研究。我们建议开发这样一种生物反应器，具有强大的模块化配置，易于使用，适用于非常广泛的生物医学研究。

DESCRIPTION (provided by applicant): We propose to develop a new bioreactor system that would radically advance biomedical and clinical studies of human cells and engineered tissues. The project would address a long standing need for culture systems that provide high biologic fidelity along with the precise environmental control, real time imaging, and adaptive operating strategies, in configurations that are easy to use by a wide range of investigators. The use of currently available bioreactor systems has significantly advanced our ability to conduct fundamental and translational research. However, major limitations remain. There is a need for bioreactors that would combine several critical requirements: (i) provision of precisely controlled 3D environments resembling those encountered in vivo, (ii) application of multiple regulatory factors (molecular, physical, cell- and matrix- derived), (iii) modular designs for high-throughput and combinatorial studies, and (iv) live imaging compatibility for real-time insight. Our laboratory has been developing bioreactors for tissue engineering for almost two decades. We propose to integrate and advance our accumulated experience and resources to develop a truly advanced new bioreactor with broad relevance to biomedical and clinical research. Three specific aims will be pursued in an integrated fashion towards the optimization and full validation of the proposed bioreactor system. Aim 1 is to develop a bench-top bioreactor platform with culture modules consisting of self-sustained cartridges with medium flow and environmental control. Aim 2 is to develop bioreactor configurations with medium perfusion and mechanical loading, suitable for studies of habitually loaded cells and tissues. Aim 3 is to develop bioreactor configuration with perfusion, electrical and mechanical stretch, suitable for studies of electromechanically active cells and tissues. The proposed system will be based on a common platform providing a set of basic functions that will be interfaced with on-line imaging, environmental control, and data acquisition. The focus of our work will be on the biological requirements of stem cell and tissue engineering research, and the implementation of radically new engineering solutions for a bioreactor design. PUBLIC HEALTH RELEVANCE (provided by applicant): Development of bioreactors that would enable studies of human cells and engineered tissues under conditions similar to those in the body, with precise environmental control and live imaging would truly advance biological and medical research. We propose to develop such a bioreactor, with robust modular configuration that is easy to use and suitable for a very broad range of biomedical studies.