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Role of Toll-Like Receptors in Atherogenesis

Toll 样受体在动脉粥样硬化形成中的作用

基本信息

批准号：
7851219
负责人：
Linda K Curtiss
金额：
$ 46.6万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7851219
关键词：
Agonist Alleles Animals Atherosclerosis Blood Vessels Bone Marrow CD14 gene CD36 gene Cells Chronic Communicable Diseases Dendritic Cells Diet Disease Disease Progression Endothelial Cells Fatty acid glycerol esters Fibroblasts Gene Deletion Genes Genetic Programming HMGB1 Protein Human Hyaluronic Acid Hyperlipidemia Immune Immune system In Vitro Infection Infectious Agent Infiltration Inflammation Inflammation Mediators Inflammatory Inflammatory Response Lesion Ligands Link Lipids Lipoproteins Low Density Lipoprotein Receptor Lymphocyte Mediating Modeling Mus Natural Immunity Natural Killer Cells Plasma Prevention Process Program Research Project Grants Publishing Reporting Risk Risk Factors Role Serum amyloid A protein Severity of illness Signal Transduction Smooth Muscle Myocytes Sterility TLR1 gene TLR2 gene TLR4 gene TLR6 gene Testing Therapeutic Intervention Time Toll-Like Receptor 1 Toll-Like Receptor 2 Toll-like receptors atherogenesis biglycan bone disorder risk feeding in vivo lipoteichoic acid macrophage macrophage stimulatory lipopeptide 2 monocyte oxidized lipid pathogen programs receptor research study response sensor

项目摘要

Atherosclerosis is a chronic inflammatory disease of the arterial wall. THis has been established by studies of specific inflammatory gene deletions in hyperlipidemic mice that influence disease severity, the Toll-like receptors (TLR) of the innate immune system, which sense pathogens and mediate cell activation, can provide an important link between infection, inflammation and atherosclerosis. We discovered that TLR2- mediated inflammation influences disease progression in low density lipoprotein receptor-deficient (LDLr-/-) mice. Proatherogenic inflammatory TLR2-mediated responses to unknown endogenous agonists are mediated by non bone marrow-derived cells including endothelial cells, smooth muscle cells and advential fibroblasts. In contrast the proatherogenic inflammatory responses to the known exogenous, synthetic TLR2 agonist, Pam3, are mediated by bone marrow-derived cells including macrophages. In Project 4 of this program project grant we will confirm that TLR2-mediated cell activation by either endogenous or exogenous TLR2 agonists is predominately proatherogenic and analyze how TLR2-mediated inflammation influences atherosclerosis. In Aim 1 we will study endogenous agonists of TLR2. We will characterize region-specific expression of TLR2 in vivo in non-bone marrow-derived cells and document the time course of the effect of TLR2 on macrophage infiltration into lesions. We will identify candidate endogenoous proatherogenic agonists and define the role of the TLR2 co-receptors, TLR1, TLR6 and CD36 in TLR2 signaling. In Aim 2 we will study exogenous agonists of TLR2. We will determine if macrophages are sufficient for mediating proatherogenic inflammation induced by defined exogenous agonists. We will define the role of the TLR2 co-receptors with known exogenous agonists. These studies will enhance our understanding of inflammatory responses in atherosclerosis and potentially identify new TLR targets for therapeutic intervention to reduce disease risk.

动脉粥样硬化是动脉壁的一种慢性炎症性疾病。这是通过研究确定的高脂血症小鼠中影响疾病严重程度的特定炎症基因缺失的研究，Toll-like 先天免疫系统的受体（TLR）可以感知病原体并介导细胞激活，可以提供感染、炎症和动脉粥样硬化之间的重要联系。我们发现 TLR2- 介导的炎症影响低密度脂蛋白受体缺陷（LDLr-/-）的疾病进展老鼠。促动脉粥样硬化炎症 TLR2 介导的对未知内源性激动剂的反应是由非骨髓源性细胞介导，包括内皮细胞、平滑肌细胞和外膜细胞成纤维细胞。相比之下，对已知的外源合成 TLR2 的促动脉粥样硬化炎症反应激动剂 Pam3 由骨髓源性细胞（包括巨噬细胞）介导。在本项目4中计划项目拨款，我们将确认 TLR2 介导的细胞激活是通过内源性或外源性的 TLR2 激动剂主要是促动脉粥样硬化，并分析 TLR2 介导的炎症如何影响动脉粥样硬化。在目标 1 中，我们将研究 TLR2 的内源性激动剂。我们将根据地区特点 TLR2 在非骨髓来源细胞体内的表达，并记录效果的时间过程 TLR2 影响巨噬细胞浸润病变。我们将确定候选的内源性致动脉粥样硬化激动剂并定义了 TLR2 共受体 TLR1、TLR6 和 CD36 在 TLR2 信号传导中的作用。目标 2 我们将研究TLR2的外源激动剂。我们将确定巨噬细胞是否足以介导由特定的外源性激动剂诱导的促动脉粥样硬化炎症。我们将定义 TLR2 的角色具有已知外源激动剂的共受体。这些研究将加深我们对动脉粥样硬化中的炎症反应并可能确定新的 TLR 治疗靶点干预以降低疾病风险。