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Assay Dev/Adapt (California)

Assay Dev/Adapt (California)
检测开发/适应（加利福尼亚州）
批准号：
8120934
负责人：
Steven J Brown
金额：
$294.99万
依托单位：
SCRIPPS RESEARCH INSTITUTE, THE
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
项目状态：
已结题
起止时间：
2010-06-01 至 2014-05-31
来源：
https://reporter.nih.gov/project-details/8120934
关键词：
AdherenceApplications GrantsAutomationBiological AssayBusiness RulesCalciumCaliforniaCatalogingCatalogsCell CountCell LineCellsChemicalsChemistryCollaborationsColorComputer softwareCritical PathwaysCyclic AMPDataData FilesData QualityDatabasesDetectionDevelopmentDevelopment PlansDimethyl SulfoxideDoseEnsureEnzymesEquipmentEvaluationFloridaFluorescein-5-isothiocyanateFluorescenceFluorescence MicroscopyFollow-Up StudiesFreezingG Protein-Coupled Receptor GenesGeneric DrugsGoalsHandHourHousingImageImage AnalysisIncubatorsInformaticsLabelLaboratoriesLactamaseLasersLengthLifeMaintenanceMeasurementMeasuresMentorsMicroinjectionsMicroscopeMicroscopyMiniaturizationMolecular and Cellular BiologyMycoplasmaNucleic AcidsOutsourcingPeptidesPerformanceProceduresProcessProductionProteinsProtocols documentationProviderPubChemQuality ControlReadinessReagentRecordsReporterReportingResearch PersonnelResourcesRoboticsRunningSamplingScanningScheduleScienceScreening procedureSecureSourceSpecificityStreamSummary ReportsSurveysSynthesis ChemistrySystemTeleconferencesTemperatureTestingTimeTimeLineTitrationsUnited States National Institutes of HealthUpdateVendorVial deviceVisualWorkWorkplaceassay developmentbasecontinuous cell linecounterscreenenzyme substratefluorescence microscopefollow-upfootinhibitor/antagonistinterestmeetingsmembermicromanipulatoroutreachrelease of sequestered calcium ion into cytoplasmresponsescale upsquare foottissue culture

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ASSAY DEVELOPMENT/ADAPTATION CORE In La Jolla, 600 square ft of laboratory space is dedicated to the MLPCN effort. The lab is configured for maximal use of space with the tissue culture room adjacent to the automation bench. The tissue culture room has two 6-foot biosafety cabinets. In addition, the PI has 2500 sq. ft lab space immediately below the assay development facility. The lab is fully equipped for cell and molecular biology and is used in assay development efforts requiring these capabilities. The tissue culture lab has a Zeiss Axiovert fluorescent microscope equipped with filters for visual detection of green fluorescence (FITC, GFP) and CCF2/4 (b-lactamase substrate) loaded cells. The ICND building has a core microscopy facility available for secondary assays (e.g. GPCR internalization) with the following equipment. ¿ Brightfield and fluorescence microscopes ¿ Dissecting stereoscope with color camera ¿ Calcium imaging automated system with micromanipulator for cell picking Olympus confocal laser-scanning microscope with 4 laser lines ¿ DeltaVision deconvolution acquisition system ¿ Spinning-disk confocal microscope (2 laser lines) with incubator chamber for live-cell fluorescence microscopy ¿ Live-cell brightfield microscope with incubator ¿ Eppendorf microinjection system on inverted fluorescence microscope ¿ MetaMorph 6.5 acquisition and image analysis software
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Assay Dev/Adapt (California)
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  • 财政年份：
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  • 负责人：
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  • 负责人：
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  • 项目类别：
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  • 财政年份：
    2006
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