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Assay Dev/Adapt (California)

Assay Dev/Adapt (California)
检测开发/适应（加利福尼亚州）
批准号：
8525451
负责人：
Steven J Brown
金额：
$179.59万
依托单位：
SCRIPPS RESEARCH INSTITUTE, THE
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
已结题
起止时间：
至 2015-05-31
来源：
https://reporter.nih.gov/project-details/8525451
关键词：
AdherenceApplications GrantsAutomationBiological AssayBusiness RulesCalciumCaliforniaCatalogingCatalogsCell CountCell LineCellsCellular biologyChemicalsChemistryCollaborationsColorComputer softwareCritical PathwaysCyclic AMPDataData FilesDetectionDevelopmentDevelopment PlansDimethyl SulfoxideDoseEnsureEnzymesEquipmentFluorescein-5-isothiocyanateFluorescenceFluorescence MicroscopyFollow-Up StudiesFreezingG Protein-Coupled Receptor GenesGeneric DrugsHandHourHousingImageImage AnalysisIncubatorsLabelLaboratoriesLactamaseLasersLengthLifeMeasurementMeasuresMicroinjectionsMicroscopeMicroscopyMolecular BiologyMycoplasmaNucleic AcidsOutsourcingPeptidesPerformanceProcessProductionProteinsProtocols documentationProviderReadinessReagentReporterReportingResearch PersonnelResourcesRunningSamplingScanningScheduleScienceSecureSourceSpecificityStreamSurveysSystemTeleconferencesTemperatureTestingTimeTimeLineTitrationsUnited States National Institutes of HealthVendorVial deviceVisualWorkassay developmentbasecontinuous cell linecounterscreenenzyme substratefluorescence microscopefollow-upfootinhibitor/antagonistmeetingsmicromanipulatorrelease of sequestered calcium ion into cytoplasmresponsescale upscreeningtissue culture

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英文摘要
ASSAY DEVELOPMENT/ADAPTATION CORE In La Jolla, 600 square ft of laboratory space is dedicated to the MLPCN effort. The lab is configured for maximal use of space with the tissue culture room adjacent to the automation bench. The tissue culture room has two 6-foot biosafety cabinets. In addition, the PI has 2500 sq. ft lab space immediately below the assay development facility. The lab is fully equipped for cell and molecular biology and is used in assay development efforts requiring these capabilities. The tissue culture lab has a Zeiss Axiovert fluorescent microscope equipped with filters for visual detection of green fluorescence (FITC, GFP) and CCF2/4 (b-lactamase substrate) loaded cells. The ICND building has a core microscopy facility available for secondary assays (e.g. GPCR internalization) with the following equipment. ¿ Brightfield and fluorescence microscopes ¿ Dissecting stereoscope with color camera ¿ Calcium imaging automated system with micromanipulator for cell picking Olympus confocal laser-scanning microscope with 4 laser lines ¿ DeltaVision deconvolution acquisition system ¿ Spinning-disk confocal microscope (2 laser lines) with incubator chamber for live-cell fluorescence microscopy ¿ Live-cell brightfield microscope with incubator ¿ Eppendorf microinjection system on inverted fluorescence microscope ¿ MetaMorph 6.5 acquisition and image analysis software
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Assay Dev/Adapt (California)
  • 批准号：
    8538721
  • 项目类别：
  • 资助金额：
    $94.88万
  • 财政年份：
    2012
  • 负责人：
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  • 依托单位：
    SCRIPPS RESEARCH INSTITUTE, THE
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  • 批准号：
    8120934
  • 项目类别：
  • 资助金额：
    $294.99万
  • 财政年份：
    2010
  • 负责人：
    Steven J Brown
  • 依托单位：
    SCRIPPS RESEARCH INSTITUTE, THE
Assay Dev/Adapt (California)
  • 批准号：
    8332831
  • 项目类别：
  • 资助金额：
    $307.9万
  • 财政年份：
    2008
  • 负责人：
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  • 依托单位：
    SCRIPPS RESEARCH INSTITUTE, THE
Development of a High Throughput Screening Galanin 3 Receptor Assay
  • 批准号：
    7172042
  • 项目类别：
  • 资助金额：
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  • 财政年份：
    2006
  • 负责人：
    Steven J Brown
  • 依托单位：
    SCRIPPS RESEARCH INSTITUTE, THE