Assay Dev/Adapt (California)

分析开发/适应核心 在拉霍亚，600平方英尺的实验室空间专门用于MLPCN的努力。实验室配置为 最大限度地利用与自动化工作台相邻的组织培养室的空间。组织培养室 有两个6英尺高的生物保险柜。 此外，PI还有2500平方米。FT实验室空间就在化验开发设施的正下方。实验室已经满了 配备了细胞和分子生物学，并用于需要这些能力的分析开发工作。 组织培养实验室有一台蔡司Axiovert荧光显微镜，配备了滤光片，用于视觉检测 绿色荧光(FITC、GFP)和CCF2/4(b-内酰胺酶底物)负载细胞。 ICND大楼有一个核心显微镜设施，可用于二次分析(例如GPCR内化) 使用以下设备。 亮场显微镜和荧光显微镜 用彩色摄像机解剖体视镜 带有微操作器的细胞拾取钙成像自动化系统 奥林巴斯四条激光谱线共聚焦激光扫描显微镜 ？DeltaVision反卷积捕获系统 带活细胞荧光培养箱的转盘式共聚焦显微镜(2条激光线路) 显微镜 带孵化器的活细胞明场显微镜 倒置荧光显微镜上的埃彭多夫微量注射系统 ？Metamorph 6.5采集和图像分析软件

ASSAY DEVELOPMENT/ADAPTATION CORE In La Jolla, 600 square ft of laboratory space is dedicated to the MLPCN effort. The lab is configured for maximal use of space with the tissue culture room adjacent to the automation bench. The tissue culture room has two 6-foot biosafety cabinets. In addition, the PI has 2500 sq. ft lab space immediately below the assay development facility. The lab is fully equipped for cell and molecular biology and is used in assay development efforts requiring these capabilities. The tissue culture lab has a Zeiss Axiovert fluorescent microscope equipped with filters for visual detection of green fluorescence (FITC, GFP) and CCF2/4 (b-lactamase substrate) loaded cells. The ICND building has a core microscopy facility available for secondary assays (e.g. GPCR internalization) with the following equipment. ¿ Brightfield and fluorescence microscopes ¿ Dissecting stereoscope with color camera ¿ Calcium imaging automated system with micromanipulator for cell picking Olympus confocal laser-scanning microscope with 4 laser lines ¿ DeltaVision deconvolution acquisition system ¿ Spinning-disk confocal microscope (2 laser lines) with incubator chamber for live-cell fluorescence microscopy ¿ Live-cell brightfield microscope with incubator ¿ Eppendorf microinjection system on inverted fluorescence microscope ¿ MetaMorph 6.5 acquisition and image analysis software