QUANTITATIVE METABOLOMICS REVEALS AN EPIGENIC BLUEPRINT FOR IRON ACQUISITION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 细菌病原体通常通过与铁获取相关的获得性基因的存在来区分。然而，特定的铁载体受体基因的存在并不能可靠地预测参与这些次级代谢物的合成和运输的复杂蛋白组合的活性。在这里，我们开发了一种基于稳定同位素稀释的新的定量代谢方法来比较与肠道定植或尿路疾病相关的大肠杆菌菌株产生的铁载体的补体。由于尿路致病性大肠埃希菌被认为在感染前存在于肠道微生物群中，我们比较了反复尿路感染患者尿液和直肠分离株中铁载体的产生。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Bacterial pathogens are frequently distinguished by the presence of acquired genes associated with iron acquisition. The presence of specific siderophore receptor genes, however, does not reliably predict activity of the complex protein assemblies involved in synthesis and transport of these secondary metabolites. Here, we have developed a novel quantitative metabolomic approach based on stable isotope dilution to compare the complement of siderophores produced by E. coli strains associated with intestinal colonization or urinary tract disease. Because uropathogenic E. coli are believed to reside in the gut microbiome prior to infection, we compared siderophore production between urinary and rectal isolates within individual patients with recurrent UTI.