QUANTITATIVE METABOLOMICS REVEALS AN EPIGENIC BLUEPRINT FOR IRON ACQUISITION

定量代谢组学揭示了铁获取的表观蓝图

• 批准号: 8361387
• 负责人: Jeffrey A Henderson
• 金额: $ 1.64万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361387
• 关键词: Complement; Escherichia coli; Funding; Genes; Grant; Individual; Infection; Intestines; Iron; Mass Spectrum Analysis; National Center for Research Resources; Patients; Principal Investigator; Production; Receptor Gene; Recurrence; Research; Research Infrastructure; Resources; Siderophores; Source; United States National Institutes of Health; Urologic Diseases; Uropathogenic E. coli; base; biomedical resource; cost; metabolomics; microbiome; novel; pathogen; protein complex; rectal; siderophore receptors; stable isotope; urinary

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Bacterial pathogens are frequently distinguished by the presence of acquired genes associated with iron acquisition. The presence of specific siderophore receptor genes, however, does not reliably predict activity of the complex protein assemblies involved in synthesis and transport of these secondary metabolites. Here, we have developed a novel quantitative metabolomic approach based on stable isotope dilution to compare the complement of siderophores produced by E. coli strains associated with intestinal colonization or urinary tract disease. Because uropathogenic E. coli are believed to reside in the gut microbiome prior to infection, we compared siderophore production between urinary and rectal isolates within individual patients with recurrent UTI.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 细菌病原体通常通过与铁获得相关的获得性基因的存在来区分。然而，特异性铁载体受体基因的存在并不能可靠地预测参与这些次级代谢产物合成和转运的复杂蛋白组装体的活性。 在这里，我们已经开发了一种新的定量代谢方法的基础上稳定同位素稀释比较铁载体的补充E。大肠杆菌菌株与肠道定植或泌尿道疾病有关。因为尿路致病性E.大肠杆菌被认为在感染前存在于肠道微生物组中，我们比较了复发性UTI患者尿液和直肠分离株之间的铁载体产生。