In Vivo Core - Interdepartmental Two-photon Imaging Center

In Vivo Core - 跨部门双光子成像中心

• 批准号: 7816797
• 负责人: David Jack Walters
• 金额: $ 10.09万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7816797
• 关键词: Age; Area; Biological Neural Networks; Brain Injuries; Cell physiology; Center Core Grants; Commit; Communities; Computer software; Core Facility; Cues; Dendrites; Development; Direct Costs; Discipline; Disease; Electrophysiology (science); Ensure; Environment; Equipment; Event; Faculty; Failure; Funding; Future; Gestures; Goals; Hand; Health; Image; Imaging technology; In Vitro; Industry; Information Dissemination; Internet; Investments; Laboratories; Letters; Life; Measures; Microscope; Microscopy; Molecular; Monitor; Movement; National Institute of Neurological Disorders and Stroke; Nervous system structure; Neurons; Neurosciences; Operative Surgical Procedures; Optics; Performance; Physics; Physiology; Property; Publications; Recruitment Activity; Regenerative Medicine; Research; Research Infrastructure; Research Personnel; Resolution; Resources; Schools; Secure; Stem cells; Structure; Surface; Synapses; Techniques; Technology; Tissues; Training; Translating; Uncertainty; United States National Institutes of Health; Wages; Water; Work; base; commercialization; cost; design; experience; improved; in vivo; insight; instrument; instrumentation; molecular imaging; nerve stem cell; nervous system disorder; programs; ranpirnase; stem cell biology; tool; two-photon; user-friendly

NU and FSM have made a strong commitment to the development of shared 2P imaging facilities. FSM has invested approximately $2.2M to purchase equipment, renovate space and hire technical staff for the three existing 2P core facilities. As part of their continuing commitment, FSM has authorized the allocation of an additional $300K for the purchase of instrumentation to create a core for in vivo 2P imaging (Core 4). This was a 'matching' commitment in the original P30 application but the money was given as a good faith gesture in spite of the failure to secure NINDS support. It is worth noting that the $2.5M figure does not reflect the school's investment in new faculty (salaries, lab renovations, start-up costs) with demonstrated expertise in 2P imaging applications (Drs. Grutzendler, Waters, Shepherd, and Osten); this investment exceeds $4M. If one adds in the investment in the last three years in faculty working in dendritic-synaptic physiology and neural stem cell biology (creating the neuroscience community in which these techniques can fluorish), the total NU commitment to this enterprise exceeds $25M. With the support called for in this proposal, we can build on this commitment by improving the operation of the existing cores as well as creating new ones for NINDS funded investigators who need these technologies to advance their research programs but cannot be accommodated at present. NU is prepared to expand their commitment if NINDS takes this step, providing additional space (Cores 5 and 6) and salary support for core staff (Drs. Wokosin, Hockberger and Core 6 staff; see accompanying commitment letters).

NU和FSM已经对共享2 P成像设施的发展做出了坚定的承诺。密克罗尼西亚联邦 投资约220万美元购买设备，翻新空间并聘请技术人员， 三个现有的2 P核心设施。作为其持续承诺的一部分，密克罗尼西亚联邦已授权分配 额外的30万美元用于购买仪器，以创建用于体内2 P成像的核心（核心 4）。这是一个'匹配'的承诺，在原来的P30应用程序，但钱是作为一个良好的信誉 尽管未能获得NINDS的支持，但这是一个姿态。值得注意的是，250万美元的数字并没有反映出 学校对新教师的投资（工资，实验室装修，启动成本）， 2 P成像应用（Grutzendler、沃茨、Shepherd和Osten博士）;投资超过400万美元。如果 其中一项是在过去三年中对树突突触生理学和神经生理学教师的投资， 干细胞生物学（创建神经科学社区，其中这些技术可以氟化），总NU 对该企业的承诺超过2500万美元。有了这项建议所要求的支持，我们可以在 通过改善现有核心的运作以及为NINDS建立新的核心， 研究人员需要这些技术来推进他们的研究计划，但无法适应 礼物如果NINDS采取这一步骤，NU准备扩大其承诺，提供额外的空间（核心 核心工作人员（Wokosin博士、Hockberger博士和核心6工作人员;见所附承诺）的薪金支助 字母）。