Microrheology techniques for pharmaceutical research

用于药物研究的微流变学技术

• 批准号: G0601775/1
• 负责人: Thomas Waigh
• 金额: $ 11.96万
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=G0601775%2F1
• 关键词: Microrheology; techniques; pharmaceutical; research

Drugs need to be formulated into medicines before they can be given to patients in a useful form. Injections are an important group of formulations and their viscoelasticity, that is, the ease in which the fluid can be deformed as it passes through the syringe, is an important consideration. However, bulk testing of the viscoelastic behaviour of injectables is often not feasible since they are available only in limited amounts. Here we will investigate new techniques in the field of microrheology, which have been demonstrated to measure the viscoelasticity of very small volumes of sample. The volumes that can be measured are so small that the technique can be applied to the measurement of the viscoelasticity of sub-cellular compartments such as the nucleus and mitochondria. Therefore, in addition to addressing the key issue of pharmaceutical formulation the work will also attempt to measure changes in the viscoelastic environment of a drug as it is internalised into a cell via a process known as endocytosis.

药物需要先配制成药物，然后才能以有用的形式给病人服用。注射剂是一组重要的配方，它们的粘弹性，即液体在通过注射器时容易变形，是一个重要的考虑因素。然而，注射剂的粘弹性特性的批量测试通常是不可行的，因为它们的数量有限。在这里，我们将研究微流变学领域的新技术，这些技术已被证明可以测量非常小体积样品的粘弹性。可测量的体积是如此之小，以至于该技术可以应用于测量亚细胞区室（如细胞核和线粒体）的粘弹性。因此，除了解决药物配方的关键问题外，该工作还将尝试测量药物通过称为内吞作用的过程内化到细胞中的粘弹性环境的变化。