Mouse Models of Pulmonary Inflammation Core

肺部炎症核心小鼠模型

• 批准号: 8005443
• 负责人: Charles Wayne Frevert
• 金额: $ 24.12万
• 依托单位: BENAROYA RESEARCH INST AT VIRGINIA MASON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-10 至 2015-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8005443
• 关键词: AIDS/HIV problem; Air; Alveolar Macrophages; Arts; Bacteria; Biocompatible Materials; Biological; Blood; Bone Marrow; Bronchoalveolar Lavage Fluid; Cells; Collection; Comparative Pathology; Complement; Computer software; Consultations; Coronary heart disease; Data; Data Quality; Development; Disease; Doctor of Philosophy; Epithelial Cells; Equipment; Fetal Liver; Fibroblasts; Genetically Engineered Mouse; Goals; Harvest; Image; Image Analysis; Individual; Infection; Inflammation; Knowledge; Laboratories; Laboratory Research; Laboratory mice; Lead; Life; Liquid substance; Lung; Lung Inflammation; Maintenance; Malignant Neoplasms; Measurement; Messenger RNA; Microscope; Modeling; Mus; Myeloid Cells; Pathology; Play; Pneumonia; Primary Cell Cultures; Procedures; Proteins; Public Health; Research; Research Personnel; Resources; Role; Slide; Specimen; Stroke; System; Technical Expertise; Technology; Time; Tissues; Training; Transgenic Animals; Transgenic Organisms; Universities; Urine; Viral; Virus; Work; animal resource; base; cellular imaging; cost; digital; experience; irradiation; macrophage; meetings; mouse model; novel therapeutics; programs; reconstitution; research study; sample collection; two-photon

The purpose of Core B - Mouse Models of Pulmonary Inflammation - is to provide a resource for investigators performing studies in mice. The Director of Core B is Charies W. Frevert, DVM, ScD, who has extensive experience in the use of mouse models of lung inflammation and infection. The Co-lnvestigator is Andrew Burich, DVM, MS who has research experience with mouse models of inflammation. To meet the needs of the program projects we have developed four goals. The first goal is to provide access to the expertise, resources, and facilities required to model pulmonary inflammation in mice. This includes access to individuals with expertise in comparative pathology and pulmonary inflammation; access to BSL-2 and BSL-3 laboratories to carry out studies on lung infection in mice; and access to a Transgenic Animal Resource Laboratory to develop genetically engineered mice proposed by the four projects. The second goal will be to standardize the mouse models of pulmonary inflammation and lung infection used by the different program projects. This is important as it will facilitate interactions between projects. The third goal is to isolate and distribute primary cell cultures obtained from mice. This includes isolation of primary cell cultures of epithelial cells grown at an air liquid interface, lung fibroblasts, bone marrow derived macrophages, and alveolar macrophages. The fourth goal will be to interact on a regular basis with Robert Vernon, PhD the Director of Core C. This interaction is important because it will insure that sample collection and submission from Core B to Core C is standardized. Drs. Frevert and Vernon will also work closely together to insure that all four projects have access to specialized imaging systems and image analysis programs required for their work. By consolidating skilled staff, facilities, and equipment in Core B and making it available to all projects, we will reduce the cost of producing high-quality data and will facilitate interactions among the different projects. Therefore, Core B will play an important role in the successful completion of work proposed in this program project.

核心B -肺部炎症的小鼠模型-的目的是提供资源， 研究人员在老鼠身上进行研究。核心B的主管是Charies W。Frevert，DVM，ScD，谁拥有 在肺部炎症和感染的小鼠模型的使用方面具有丰富的经验。共同调查员是 Andrew Burich，DVM，MS，具有炎症小鼠模型的研究经验。满足 项目的需要，我们制定了四个目标。第一个目标是提供进入 在小鼠中建立肺部炎症模型所需的专业知识、资源和设施。这包括访问 具有比较病理学和肺部炎症专业知识的个人;获得BSL-2和 BSL-3实验室进行小鼠肺部感染研究;获得转基因动物 资源实验室提出开发基因工程小鼠的四个项目。第二个目标 将标准化的小鼠模型用于肺部炎症和肺部感染的不同 方案项目。这一点很重要，因为它将促进项目之间的互动。第三个目标是 分离和分配从小鼠获得原代细胞培养物。这包括分离原代细胞培养物 在气液界面生长的上皮细胞、肺成纤维细胞、骨髓来源的巨噬细胞，以及 肺泡巨噬细胞第四个目标将是定期与罗伯特弗农博士互动， 核心C的领导者这种互动很重要，因为它将确保样本收集和提交 从核心B到核心C是标准化的。Frevert和弗农博士也将密切合作，以确保 所有四个项目都可以使用专门的成像系统和图像分析程序， 工作通过整合核心B中的熟练员工、设施和设备，并使其可用于所有项目， 我们将降低生产高质量数据的成本，并将促进不同部门之间的互动， 项目因此，核心B将在成功完成本报告中提出的工作方面发挥重要作用。 程序项目。