Animal Core
动物核心
基本信息
- 批准号:7790043
- 负责人:
- 金额:$ 28.99万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-08-01 至 2015-07-31
- 项目状态:已结题
- 来源:
- 关键词:AcuteAcute PneumoniaAnimalsApoE knockout mouseApolipoprotein EArterial Fatty StreakAtherosclerosisBacterial InfectionsBloodBlood PlateletsBlood specimenBone MarrowBone Marrow TransplantationBreedingCellsChlamydophila pneumoniaeChronicCollecting CellCommunitiesDevelopmentEndothelial CellsFoam CellsGene DeletionGoalsIL1R1 geneImageImmuneImmune responseIn VitroInfectionInflammationInflammatoryInflammatory ResponseInterleukin-1Interleukin-1 ReceptorsKnock-outKnockout MiceLettersLipidsMagnetic Resonance ImagingMegakaryocytesMouse StrainsMusNatural ImmunityOralPneumoniaPorphyromonas gingivalisResearchResearch Project GrantsRobin birdRoleSignal PathwaySignal TransductionSpecificityTLR2 geneTissuesTransgenic Miceatherothrombosisbone lossin vivomacrophagemouse modelnovelosteoclastogenesispathogenprogramsreceptor expressionresponse
项目摘要
The purpose of the Animal Core of this Program Project is to support the four research projects by
maintaining and breeding mice, collecting cells, tissues and blood from mice, performing bacterial infection
with Porphyromonas gingivalis and Chlamydia pneumoniae, and performing in vivo imaging studies by MRI /
MRA. To define cell specificity of IL-lp signaling in the development of inflammatory atherosclerotic plaque,
the Animal Core will perform bone marrow transplantation to produce bone marrow chimeric mice in which
either the donor cells or the recipient mice are IL-1p KO, and challenge these mice with C. pneumoniae or
P. gingivalis. The Animal Core will generate two novel transgenic mice, in which TLR2 or IL-1 receptor (IL-
1R) expression is targeted specifically to endothelial cells. These mice will be used to examine endothelial
cell TLR2 or IL-IR signaling in inflammatory responses to P. gingivalis and C. pneumoniae infection. We
currently maintain some of these KO mice in our mouse colony, including the ApoE, TLR2, IL-1 R single KO
mice as well as ApoE/TLR2 double KO mice. We will obtain IL-ip, LXRa, LXRp single KO mice, and
LXRa/p double KO mice.We will cross KO mice to generate double KO mice: ApoE x LXRa, ApoE x LXRp,
ApoE X IL-IR, and ApoE x IL-1 p. To support the four Projects of the Program, we propose the following Aims
for the Animal Core: AIM 1. Maintain and breed knockout mouse lines. AIM 2.Obtain cell, tissue and blood
samples from mice for analysis by each project. AIM 3. Perform infection of P. gingivalis or C. pneumoniae,
generate bone marrow chimeric mice by bone marrow transplantation, and perform MRI / MRA for each
project. AIM 4.Construct novel transgenic mice strains in which TLR2 or IL-IR expression is targeted to
endothelial cells.
RELEVANCE
本计划项目的动物核心的目的是支持四个研究项目,
小鼠的饲养繁殖,收集小鼠的细胞、组织和血液,进行细菌感染,
牙龈卟啉单胞菌和肺炎衣原体,并通过MRI /MRI进行体内成像研究。
MRA。为了确定炎性动脉粥样硬化斑块发展中IL-1 p信号传导的细胞特异性,
动物中心将进行骨髓移植以产生骨髓嵌合小鼠,其中
无论是供体细胞还是受体小鼠都是IL-1 β KO,并用C.肺炎或
P.牙龈炎动物中心将产生两个新的转基因小鼠,其中TLR 2或IL-1受体(IL-1 R)
1 R)表达特异性靶向内皮细胞。这些小鼠将用于检查内皮细胞
细胞TLR 2或IL-1 R信号转导对牙龈卟啉单胞菌和C.肺炎感染。我们
目前在我们的小鼠群体中保留了一些这些KO小鼠,包括ApoE、TLR 2、IL-1 R单KO
小鼠以及ApoE/TLR 2双KO小鼠。我们将获得IL-1 β、LXR α、LXR β单基因敲除小鼠,
我们将杂交KO小鼠以产生双KO小鼠:ApoE x LXRa,ApoE x LXRp,
ApoE X IL-1 p和ApoE X IL-1 R。为了支持该计划的四个项目,我们提出了以下目标
动物核心:AIM 1。保持和繁殖敲除小鼠品系。目的2.获取细胞、组织和血液
每个项目从小鼠身上采集样本进行分析。AIM 3.进行牙龈卟啉单胞菌或C.肺炎,
通过骨髓移植产生骨髓嵌合体小鼠,并对每个小鼠进行MRI / MRA,
项目目的4.构建靶向表达TLR 2或IL-1 R的新型转基因小鼠品系,
内皮细胞
相关性
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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LEE Mark WETZLER其他文献
LEE Mark WETZLER的其他文献
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{{ truncateString('LEE Mark WETZLER', 18)}}的其他基金
Gonococcal Vaccine Evaluation in a Humanized Mouse Model
人源化小鼠模型中的淋球菌疫苗评估
- 批准号:
9389653 - 财政年份:2013
- 资助金额:
$ 28.99万 - 项目类别:
Gonococcal Vaccine Evaluation in a Humanized Mouse Model
人源化小鼠模型中的淋球菌疫苗评估
- 批准号:
8584887 - 财政年份:2013
- 资助金额:
$ 28.99万 - 项目类别:
Gonococcal Vaccine Evaluation in a Humanized Mouse Model
人源化小鼠模型中的淋球菌疫苗评估
- 批准号:
8868920 - 财政年份:2013
- 资助金额:
$ 28.99万 - 项目类别:
Gonococcal Vaccine Evaluation in a Humanized Mouse Model
人源化小鼠模型中的淋球菌疫苗评估
- 批准号:
8702079 - 财政年份:2013
- 资助金额:
$ 28.99万 - 项目类别:
Boston University Inflammatory Disorders Training Grant
波士顿大学炎症性疾病培训补助金
- 批准号:
8860100 - 财政年份:2011
- 资助金额:
$ 28.99万 - 项目类别:
Effect of Neisserial Porin in Immune Cell Apoptosis
奈瑟氏球菌孔蛋白对免疫细胞凋亡的影响
- 批准号:
6758513 - 财政年份:2002
- 资助金额:
$ 28.99万 - 项目类别:
Effect of Neisserial Porin in Immune Cell Apoptosis
奈瑟氏球菌孔蛋白对免疫细胞凋亡的影响
- 批准号:
6913540 - 财政年份:2002
- 资助金额:
$ 28.99万 - 项目类别:
Effect of Neisserial Porin in Immune Cell Apoptosis
奈瑟氏球菌孔蛋白对免疫细胞凋亡的影响
- 批准号:
6544274 - 财政年份:2002
- 资助金额:
$ 28.99万 - 项目类别:
Effect of Neisserial Porin in Immune Cell Apoptosis
奈瑟氏球菌孔蛋白对免疫细胞凋亡的影响
- 批准号:
6645684 - 财政年份:2002
- 资助金额:
$ 28.99万 - 项目类别:
Effect of Neisserial Porin in Immune Cell Apoptosis
奈瑟氏球菌孔蛋白对免疫细胞凋亡的影响
- 批准号:
7080405 - 财政年份:2002
- 资助金额:
$ 28.99万 - 项目类别:
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